Anti-TSPAN31 Polyclonal Antibody for WB, ELISA - Q12999

Core Product Specifications and Parameters

Biological Background: Tetraspanin-31 Function and Localization

• TSPAN31 (Tetraspanin-31) is a member of the tetraspanin family, characterized by four transmembrane domains.
• It is also known as Sarcoma-amplified sequence (SAS), originally identified due to gene amplification in human sarcomas.
• The protein localizes to the cell membrane (UniProt evidence at transcript level).
• It is a glycoprotein and contains transmembrane helices, typical of tetraspanins involved in cell adhesion, motility, and signaling.
• Predicted molecular weight is approximately 23 kDa.
• Tetraspanins often form complexes with other membrane proteins, though specific partners for TSPAN31 are not well characterized.

Experimental Guidance and Technical Tips

• This polyclonal antibody was raised against a recombinant fusion protein corresponding to amino acids 95-175 of human TSPAN31; verify specificity using positive and negative controls.
• For Western blot (WB), the predicted molecular weight is ~23 kDa. Include an appropriate loading control and validate detection in lysates from human, mouse, or rat tissues/cell lines.
• For ELISA applications, check optimal coating concentrations and consider pairing with a matched detection antibody if developing a sandwich assay.
• Cross-reactivity with human, mouse, and rat has been reported; confirm reactivity in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-TSPAN31 polyclonal antibody supports cancer and membrane protein studies, and through CamelBio you can source validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw materials to accelerate your assay development.