Anti-Tspan18 Polyclonal Antibody for WB, ELISA - Q80WR1

Core Product Specifications and Parameters

Biological Background: Tspan18 Function and Localization

• Tetraspanin-18 (Tspan18), also known by aliases 6720430O15 and 2610042G18Rik, is a member of the tetraspanin family of transmembrane proteins.
• It plays a role in the cell surface localization of ORAI1, a pore-forming subunit of store-operated calcium channels, thereby participating in the regulation of Ca2+ signaling.
• Tspan18 is implicated in the release of von Willebrand factor (VWF) from endothelial cells in response to inflammatory stimuli.
• Localized to the membrane, Tspan18 is a multi-pass membrane protein with several transmembrane helices.
• Tissue-specific expression: highly expressed in lung (PubMed:15489334) and expressed in adult heart, artery, and vein (PubMed:32694189). Related references: PMID:15489334 PMID:32694189
• Post-translational modifications include N-linked glycosylation (glycoprotein).

Experimental Guidance and Technical Tips

• This polyclonal antibody is raised against a synthetic peptide corresponding to amino acids 149–248 of mouse Tspan18. Peptide blocking experiments can be used to confirm specificity in your assay system.
• For Western blot, the predicted molecular weight of Tspan18 is approximately 28 kDa (UniProt: 27.8 kDa). A band migrating between 25–30 kDa is expected; consider using a protein ladder with appropriate resolution.
• For ELISA, the antibody may be used as a capture or detection reagent. Optimal coating concentrations and detection dilutions should be determined empirically.
• Given its membrane localization, enrichment of membrane fractions or immunoprecipitation prior to WB may improve detection.
• Cross-reactivity has been observed with mouse and rat Tspan18. Due to high sequence conservation, reactivity with other species (e.g., human) is plausible but should be validated in the target sample.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research labs to supply validated IVD raw materials, including the Anti-Tspan18 Polyclonal Antibody for investigational use in calcium signaling and endothelial cell biology. As a comprehensive sourcing bridge, we offer a portfolio of optimized antibodies, ancillary reagents, and rare target raw material procurement services to accelerate your assay development from concept to clinic. For bulk orders or customized sourcing needs, our team provides tailored support to streamline your supply chain.