Anti-TSNAX Rabbit Polyclonal Antibody for WB, ELISA - Q99598

Core Product Specifications and Parameters

Biological Background: TSNAX Function and Localization

• TSNAX, also known as Translin-associated protein X or TRAX, is a protein that functions in combination with translin (TSN).
• The TSNAX/TSN complex acts as an endonuclease involved in the activation of the RNA-induced silencing complex (RISC), playing a role in RNA interference pathways.
• TSNAX may have a role in spermatogenesis, as suggested by its association with developmental processes.
• Subcellular localization studies indicate TSNAX is found in the cytoplasm, particularly in the perinuclear region, as well as in the Golgi apparatus and nucleus.
• The protein contains DNA-binding domains and requires magnesium as a cofactor, consistent with its nuclease activity.
• Post-translational modifications include isopeptide bond formation and ubiquitination (Ubl conjugation), which may regulate its function or stability.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein covering amino acids 1-290 of human TSNAX; this full‐length fragment may generate polyclonal antibodies recognizing multiple epitopes, which can enhance detection sensitivity but may require careful blocking to minimize non‐specific binding.
• For Western blot (WB), expect a band at approximately 33 kDa in lysates from human, mouse, or rat cells expressing TSNAX. Consider using a positive control such as HeLa or testis tissue lysate, where TSNAX is known to be expressed.
• For ELISA, the recombinant immunogen can serve as a positive coating antigen; validate assay conditions (coating concentration, blocking, and secondary antibody dilution) using a known TSNAX-containing sample.
• Cross‐reactivity with mouse and rat has been reported; however, sequence homology should be checked if using for non‐human species to ensure epitope conservation. Verify in the relevant sample system before quantitative applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a streamlined procurement channel for high‐quality IVD raw materials, including validated antibodies like this anti‐TSNAX polyclonal antibody. For projects targeting RNA interference, spermatogenesis, or nucleases, we can supply complementary antibody pairs, optimized monoclonal/polyclonal reagents, and bulk ancillary reagents to support your development pipeline. Contact us to access rare‐target sourcing and tailored technical consultation—bridging your concept to clinic with efficiency.