Anti-TSLP Rabbit Polyclonal Antibody for WB, IF/ICC, and ELISA - Q969D9

Core Product Specifications and Parameters

Biological Background: TSLP Function and Localization

• TSLP (Thymic stromal lymphopoietin) is a cytokine encoded by the TSLP gene (GeneID: 85480). Alternative splicing produces at least two isoforms, with isoform 1 expressed in tissues like heart, liver, and prostate, while isoform 2 is predominant in keratinocytes of oral mucosa, skin, and salivary glands, and is secreted into saliva.
• The primary function of TSLP is to induce the release of T-cell-attracting chemokines from monocytes and to enhance the maturation of CD11c+ dendritic cells, key steps in initiating adaptive immune responses.
• TSLP can directly activate mast cells, thereby promoting allergic inflammation, suggesting a role in conditions such as asthma and atopic dermatitis.
• Additionally, TSLP may function as an antimicrobial peptide in the oral cavity and on the skin, contributing to innate immune defense at mucosal and epithelial surfaces.
• As a secreted protein, TSLP contains a signal peptide and is modified by glycosylation, with the mature protein having a molecular weight of approximately 18.1 kDa.
• The protein structure includes disulfide bonds that stabilize its conformation, which is critical for its cytokine activity.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 29-159) covers the majority of the mature TSLP protein, so the antibody is likely to detect the secreted cytokine.
• For Western blot (WB), a specific band at approximately 18 kDa is expected; consider optimizing sample preparation to preserve disulfide bonds.
• In IF/ICC, since TSLP is secreted, staining may require optimized fixation and permeabilization protocols; co-staining with an organelle marker could help confirm localization.
• For ELISA, the antibody can be tested as both capture and detection reagent; pairing with a matched monoclonal antibody may improve sensitivity.
• Cross-reactivity with mouse and rat TSLP allows for translational studies in rodent models of allergy and inflammation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials, including validated antibody pairs, bulk ancillary reagents, and rare target sourcing. This anti-TSLP polyclonal antibody supports studies on allergic inflammation and dendritic cell biology, and CamelBio can assist with customized sourcing for your diagnostic development needs.