Anti-TSLP Rabbit Monoclonal Antibody for WB, IHC, IF, ELISA - Q969D9

Core Product Specifications and Parameters

Biological Background: TSLP Function and Localization

• Thymic stromal lymphopoietin (TSLP) is a cytokine belonging to the IL-2 family, encoded by the TSLP gene. It exists in multiple isoforms due to alternative splicing; isoform 1 is widely expressed, while isoform 2 predominates in oral mucosa, skin, and salivary glands.
• TSLP induces the release of T-cell-attracting chemokines from monocytes and strongly promotes the maturation of CD11c(+) dendritic cells, linking innate and adaptive immunity.
• Through direct activation of mast cells, TSLP plays a key role in initiating and perpetuating allergic inflammatory responses, including atopic dermatitis and asthma.
• TSLP also functions as an antimicrobial peptide in the oral cavity and on the skin, suggesting a dual role in immune defense and barrier homeostasis.
• The protein is secreted and contains characteristic features such as a signal peptide, disulfide bonds, and glycosylation sites, as indicated by its UniProt keywords.
• Subcellular localization is primarily extracellular after secretion, consistent with its role as a paracrine/endocrine signaling molecule.
• Tissue expression is broad for isoform 1 (heart, liver, prostate, etc.), while isoform 2 is restricted to keratinocytes in mucosal and epidermal barriers, with secretion into saliva.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 29-159 of human TSLP, covering the mature secreted region; this region is relatively conserved in mouse, supporting the observed cross-reactivity.
• For Western blotting, the predicted molecular weight is ~18 kDa; reducing and denaturing conditions are recommended, and a low-percentage acrylamide gel may improve resolution of this small protein.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC, IF-P), optimize antigen retrieval and permeabilization steps, as the target is secreted and may be extracellular.
• When performing ELISA, ensure capture and detection antibodies recognize native conformations; this recombinant antibody may serve as a detection reagent after validation with the appropriate standard.
• Always include positive and negative controls: for human samples, stimulated dendritic cells or keratinocytes; for mouse, tissues known to express TSLP.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a specialized provider of diagnostic raw materials, offering a one-stop sourcing platform for IVD manufacturers, research laboratories, and clinical institutions. For cytokine targets like TSLP, CamelBio supplies validated antibody pairs, bulk monoclonal and polyclonal antibodies, and ancillary reagents to streamline assay development. Leverage our integrated procurement and technical support to accelerate your TSLP-related immunoassay and biomarker research projects from concept to clinic.