Anti-TSHZ3 Rabbit Polyclonal Antibody for WB, ELISA - Q63HK5

Core Product Specifications and Parameters

Biological Background: TSHZ3 Function and Localization

• TSHZ3 (Teashirt homolog 3), also known as Zinc finger protein 537, is a transcriptional regulator encoded by the TSHZ3 gene, belonging to the teashirt zinc finger family.
• Functions as a transcriptional repressor by associating with the chromatin near the CASP4 transcriptional start site(s) and recruiting histone deacetylases HDAC1 and HDAC2 to inhibit CASP4 expression. Related references: PMID:19343227
• Plays a critical role in neurodevelopment: it regulates the differentiation and maintenance of neurons controlling respiratory rhythm and airflow, including nucleus ambiguus motoneurons, and is essential for postnatal survival.
• Involved in smooth muscle differentiation in the proximal ureter by up-regulating myocardin, thereby directing the expression of smooth muscle cell-specific genes (By similarity).
• Subcellular localization encompasses the nucleus, and it is also detected in cell projections, specifically growth cones, suggesting roles in neurite outgrowth or guidance.
• Tissue expression is prominent in the brain, particularly in the fetal neocortex; notably, its levels are strongly reduced in post-mortem brains of elderly subjects with Alzheimer disease, implying a potential link to neurodegeneration.
• Structurally, TSHZ3 contains a homeobox domain, multiple zinc finger motifs, a coiled-coil region, and undergoes phosphorylation, consistent with its roles in DNA binding, protein interactions, and signal-responsive regulation.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant protein covering amino acids 791–890 of human TSHZ3, a C-terminal region. This region may contain a unique epitope; verify its accessibility in the experimental context.
• For Western blotting, a protein band at ~119 kDa is expected. Use standard high-molecular-weight transfer conditions (e.g., 10% SDS-PAGE, wet transfer) and consider optimizing blocking and antibody dilutions (e.g., 1:500–1:2000) for your sample.
• For ELISA applications, test direct or indirect formats with coating antigen at 1–10 µg/mL and primary antibody dilutions between 1:1000 and 1:5000. Validate specificity using positive (e.g., TSHZ3-overexpressing lysates) and negative controls.
• As the antibody shows cross-reactivity with mouse TSHZ3, it can be used for comparative studies; however, validate in the relevant mouse tissue or cell line to ensure consistent performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and researchers from discovery to clinical application. This anti-TSHZ3 antibody exemplifies our expertise in providing high-quality research tools for gene regulation, neuroscience, and developmental biology. Beyond individual antibodies, we supply validated antibody pairs, bulk polyclonal and monoclonal antibodies, and custom sourcing for rare targets, ensuring seamless procurement for your assay development needs.