Anti-TSHR Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P16473

Core Product Specifications and Parameters

Biological Background: TSHR Function and Localization

• Thyrotropin receptor (TSHR, also designated TSH-R, LGR3, CHNG1, hTSHR-I) is a glycoprotein receptor encoded by the TSHR gene; alternative splicing produces multiple isoforms.
• Functions as the primary receptor for thyroid-stimulating hormone (TSH) and thyrostimulin (GPHA2:GPHB5), mediating critical thyroid hormone regulation. Related references: PMID:11847099 PMID:12045258
• Upon ligand binding, TSHR couples to G(s) proteins, stimulating adenylate cyclase to generate cyclic AMP (cAMP), which then activates protein kinase A (PKA). Related references: PMID:11847099 PMID:35940205 PMID:35940204
• Downstream PKA signaling phosphorylates key effectors such as thyroid peroxidase (TPO) and the sodium/iodide symporter (NIS), driving thyroid hormone biosynthesis and secretion, and controlling thyroid cell metabolism. Related references: PMID:27638195
• The receptor localizes predominantly to the cell membrane, with enrichment at the basolateral surface in polarized thyroid epithelial cells.
• Expression is highly specific to the thyroid gland, detected at the protein level in thyroid cells and tissues.
• Post-translational modifications include glycosylation, disulfide bond formation, and sulfation, which are critical for receptor stability and function.
• Mutations and variants in TSHR are associated with congenital hypothyroidism and other thyroid disorders, underscoring its clinical importance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 21–253 of the human TSHR extracellular domain; thus, the antibody may preferentially recognize the ectodomain and potentially detect shed receptor fragments.
• For western blotting, consider probing lysates from human thyroid cell lines (e.g., Nthy-ori 3-1) or recombinant TSHR-overexpressing cells; a band near 87 kDa is anticipated, but glycosylation may cause minor shifts.
• In immunohistochemistry (IHC-P), validate antibody performance with appropriate antigen retrieval and isotype controls; thyroid tissue sections serve as a positive control.
• Evaluate cross-reactivity with mouse and rat samples in the intended application before large-scale studies.
• Polyclonal antibodies may exhibit lot-to-lot variability; we recommend titrating and validating each new lot in your assay system.

CamelBio: Your One-Stop Sourcing Bridge

As your trusted one-stop IVD raw-material partner, CamelBio supplies high-quality antibodies, antigens, and ancillary reagents to support endocrinology and thyroid disorder research. Our TSHR polyclonal antibody is part of a broader portfolio that includes validated antibody pairs, optimized monoclonal antibodies, and bulk reagents, streamlining your diagnostic development from discovery to clinical application.