Anti-TSG101/VPS23 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q99816

Core Product Specifications and Parameters

Biological Background: TSG101/VPS23 Function and Localization

• TSG101 (Tumor susceptibility gene 101 protein), also known as ESCRT-I complex subunit TSG101, is a component of the ESCRT-I complex, which regulates vesicular trafficking by binding ubiquitinated cargo proteins and sorting them into multivesicular bodies (MVBs).
• Acts as a linker between ESCRT-0 and ESCRT-I complexes, essential for the MVB pathway and lysosomal degradation of transmembrane proteins.
• Required for completion of cytokinesis; interacts with CEP55 at the midbody ring, playing a role in cell division.
• Involved in the budding of many enveloped viruses by interacting with viral late-budding motifs (P-[ST]-A-P), crucial for viral particle release from host cells.
• Required for the exosomal release of SDCBP, CD63, and syndecan. Related references: PMID:22660413
• May also participate in the extracellular release of microvesicles that differ from exosomes. Related references: PMID:22315426
• Subcellular localization includes cytoplasm, early and late endosome membranes, centrosome, midbody ring, and nucleus.
• Tissue expression is broad: heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas. Post-translational modifications include phosphorylation, acetylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from amino acids 291–390 of human TSG101, located in the C-terminal region. This region is relatively conserved; cross-reactivity with mouse and rat TSG101 has been observed in manufacturer testing. Validate specificity in your model system.
• Predicted molecular weight: ~44 kDa. In WB, a band near 44 kDa is expected; additional bands may appear due to post-translational modifications or isoforms. Use appropriate loading controls.
• For IF/ICC, the antibody may detect TSG101 at various subcellular locations (endosomes, midbody, nucleus). Optimize fixation and permeabilization conditions for your specific cell type and target compartment.
• In ELISA, the antibody can be used for peptide or protein detection. Titrate antibody concentration and consider using a blocking buffer to reduce background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to support your diagnostic and research development. This anti-TSG101/VPS23 polyclonal antibody is suitable for studies on exosome biology, viral budding, and cell cycle regulation. Contact us for tailored supply solutions.