Anti-TSG101/VPS23 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q99816

Core Product Specifications and Parameters

Biological Background: TSG101/VPS23 Function and Localization

• TSG101 (Tumor susceptibility gene 101 protein), also known as VPS23, is a core component of the ESCRT-I complex, which regulates vesicular trafficking by binding ubiquitinated cargo and mediating sorting into multivesicular bodies (MVBs).
• It plays a critical role in cytokinesis by interacting with CEP55 at the midbody, ensuring proper cell division.
• TSG101 acts as a negative growth regulator and may be involved in cell growth and differentiation.
• The protein is hijacked by many enveloped viruses (e.g., HIV-1) via interaction with late-budding motifs (P-[ST]-A-P) to facilitate viral particle budding and release.
• TSG101 is required for the exosomal release of SDCBP, CD63, and syndecan, and may also participate in non-exosomal microvesicle release. Related references: PMID:22660413 PMID:22315426
• Subcellular localization includes cytoplasm, early and late endosome membranes, centrosome, midbody ring, and nucleus.
• Ubiquitously expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitin conjugation; alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• For Western blot, the expected band is approximately 44 kDa. Consider using whole-cell lysates from human, mouse, or rat samples.
• For immunofluorescence/ICC, permeabilization is required to detect cytoplasmic and endosomal pools. Validate fixation and permeabilization conditions in your relevant cell line.
• ELISA may be used for peptide-specific detection; validate assay conditions with the immunizing peptide as a positive control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw material sourcing, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and support for rare target raw material procurement. The TSG101/VPS23 rabbit polyclonal antibody is part of our portfolio covering cell biology and virology research, ensuring diagnostic developers have reliable access to tools for studying endosomal trafficking and viral budding pathways.