Anti-TSFM Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P43897

Core Product Specifications and Parameters

Biological Background: Elongation Factor Ts, Mitochondrial Function and Localization

• Encoded by the TSFM gene, also known as EFTS or EFTSMT, producing multiple isoforms via alternative splicing.
• Catalyzes GDP to GTP exchange on EF-Tu and participates in mitochondrial translation elongation. Related references: PMID:27677415
• Localized predominantly in the mitochondrial matrix, consistent with its role in mitochondrial protein synthesis.
• Ubiquitously expressed across human tissues, with highest levels in skeletal muscle, liver, and kidney.
• Mutations in TSFM are linked to combined oxidative phosphorylation deficiency and cardiomyopathy, classifying it as a mitochondrial disease gene.
• Identified as a phosphoprotein through proteomic studies, suggesting post-translational regulation.
• Predicted molecular weight of approximately 35.4 kDa, with observed band around 35 kDa.

Experimental Guidance and Technical Tips

• The immunogen covers the C-terminal region (aa 245–346), which is conserved in human, mouse, and rat, supporting cross-reactivity data.
• For Western blot, consider using mitochondrial-enriched lysates to improve detection sensitivity; a band near 35 kDa is expected.
• In immunocytochemistry, co-staining with mitochondrial markers (e.g., Tom20) is recommended to confirm subcellular localization.
• For ELISA, recombinant TSFM protein can serve as a standard; optimize coating and detection conditions for your assay.
• Always validate the antibody in your own experimental system, as results may vary depending on sample preparation and protocol.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement by providing validated antibodies, bulk ancillary reagents, and rare target sourcing under one roof. Our TSFM polyclonal antibody supports mitochondrial translation and disease research, and we offer customized supply solutions—from optimized antibody pairs to technical consultation—to help diagnostics developers move efficiently from concept to clinic.