Anti-TSEN54 Rabbit Polyclonal Antibody for WB, ELISA - Q7Z6J9

Core Product Specifications and Parameters

Biological Background: TSEN54 Function and Localization

• TSEN54 encodes a non-catalytic subunit of the tRNA-splicing endonuclease complex, which recognizes and cleaves splice sites in precursor tRNAs to remove introns.
• The endonuclease complex cleaves pre-tRNA at 5' and 3' splice sites, generating introns and two tRNA half-molecules with 2',3' cyclic phosphate and 5'-OH termini.
• Besides tRNA processing, TSEN54 participates in mRNA processing through interactions with pre-mRNA 3'-end processing factors, linking pre-tRNA splicing to mRNA 3'-end formation.
• Human TSEN54 localizes predominantly to the nucleus and nucleolus, consistent with its role in RNA processing.
• Alternative splicing of TSEN54 yields multiple isoforms, and the protein undergoes post-translational modifications including acetylation, methylation, and phosphorylation.
• Mutations in TSEN54 are associated with autosomal recessive pontocerebellar hypoplasia (PCH), a neurodegenerative disorder characterized by structural defects of the cerebellum and pons.
• The protein is also known by aliases PCH4, PCH5, PCH2A, sen54, and SEN54L, reflecting its gene family and disease connections.
• TSEN54 is broadly expressed but expected in tissues with high nuclear activity; cross-reactivity includes human, mouse, and rat.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 300 amino acids of human TSEN54 (isoform NP_997229.2); thus, the antibody may detect multiple splice variants if the epitope is conserved.
• For Western blotting, a band near 59 kDa is expected; consider using nuclear or whole-cell lysates from human, mouse, or rat cells as positive controls.
• In ELISA applications, this polyclonal antibody could serve as a capture or detection reagent; appropriate pairing should be empirically validated.
• Given TSEN54's nuclear localization, subcellular fractionation or overexpression may enhance detection sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials and custom sourcing solutions. For tRNA processing and neurodegeneration studies, we offer validated antibodies like this TSEN54 polyclonal antibody, along with complementary reagents including recombinant proteins, ELISA kits, and ancillary biochemicals. Our portfolio is designed to accelerate assay development and biomarker validation from discovery through clinical application.