Anti-TSEN2 Polyclonal Antibody for WB, ELISA - Q8NCE0

Core Product Specifications and Parameters

Biological Background: TSEN2 Function and Localization

• TSEN2 encodes the tRNA-splicing endonuclease subunit Sen2, a catalytic component of the tRNA-splicing endonuclease complex.
• The complex cleaves pre-tRNA at 5' and 3' splice sites to remove introns, generating tRNA half-molecules with 2',3'-cyclic phosphate and 5'-OH termini.
• TSEN2 also participates in mRNA processing through association with pre-mRNA 3'-end processing factors, linking tRNA splicing to mRNA 3'-end formation.
• Two isoforms exist: isoform 1 likely carries the active site for 5'-splice site cleavage; isoform 2 retains endonucleolytic activity but cannot properly cleave pre-tRNAs.
• Subcellular localization is nuclear, with presence in the nucleolus.
• Both isoforms are widely expressed at very low levels across tissues.
• Keywords associated include neurodegeneration and pontocerebellar hypoplasia, highlighting disease relevance.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1-220 of human TSEN2 (N-terminal region), which may recognize both isoforms if the sequence is conserved.
• For Western blot (WB), the predicted molecular weight is approximately 53 kDa; ensure adequate separation and transfer conditions.
• For ELISA, consider using recombinant protein or peptide as coating antigen and validate in the relevant sample system.
• Due to cross-reactivity with mouse and rat, this antibody can be used for studies in these model organisms; verify staining patterns with appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For RNA processing and tRNA biology research, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Our supply chain ensures seamless procurement for your development needs.