Anti-TSC22D3 Polyclonal Antibody for WB, ELISA - Q99576

Core Product Specifications and Parameters

Biological Background: TSC22D3 Function and Localization

• Protects T-cells from IL2 deprivation-induced apoptosis by inhibiting FOXO3A transcriptional activity, leading to downregulation of the pro-apoptotic factor BCL2L11 (PubMed:15031210). Related references: PMID:15031210
• Plays an anti-inflammatory and immunosuppressive role in macrophages downstream of glucocorticoid and IL10 signaling (PubMed:12393603). Related references: PMID:12393603
• In T-cells, inhibits anti-CD3-induced NFKB1 nuclear translocation and DNA-binding activity, suppressing NF-κB-driven gene expression (PubMed:11468175). Related references: PMID:11468175
• In vitro, suppresses AP-1 transcription factor complex DNA-binding activities, further contributing to immunosuppression.
• Inhibits myogenic differentiation and mediates anti-myogenic effects of glucocorticoids by binding and regulating MYOD1 and HDAC1 transcriptional activity, resulting in reduced MYOG expression.
• Localizes to both cytoplasm and nucleus, enabling its dual role in cytoplasmic signaling and nuclear transcriptional regulation.
• Widely expressed across tissues; in non-lymphoid tissues, constitutive expression is predominantly from macrophages. Highest expression in brain, lung, spleen, and skeletal muscle (PubMed:11313722, PubMed:12393603). Related references: PMID:11313722 PMID:12393603
• Subject to post-translational modifications such as acetylation and phosphorylation, and alternative splicing generates multiple protein isoforms.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 58–134, a region likely to be solvent-exposed and conserved; confirm epitope accessibility in your specific assay format.
• For Western blot, a protein band around 15 kDa is expected; optimize gel percentage (e.g., 12–15% SDS-PAGE) and transfer conditions for low-molecular-weight proteins.
• Validate cross-reactivity in non-human species if needed, as specificity has only been confirmed for human TSC22D3.
• As a polyclonal antibody, it may recognize multiple epitopes; for sandwich ELISA, consider pairing with a compatible monoclonal antibody for optimal sensitivity.
• Test performance in your target sample type (cell lysates, tissue homogenates) under your standard conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive sourcing platform for IVD raw materials, offering high-quality antibodies like this anti-TSC22D3 polyclonal antibody for apoptosis, inflammation, and glucocorticoid signaling research. Beyond individual reagents, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material procurement to support every stage of IVD development. Partner with CamelBio to streamline your supply chain and accelerate your diagnostic innovation.