Anti-TSC22D3 Polyclonal Antibody for IF/ICC, ELISA - Q99576

Core Product Specifications and Parameters

Biological Background: TSC22D3 Function and Localization

• TSC22D3 (also known as GILZ, DIP, DSIPI, TSC-22R) is a glucocorticoid-induced leucine zipper protein belonging to the TSC-22 domain family. It is encoded by the TSC22D3 gene and has a predicted molecular weight of approximately 14.8 kDa.
• The protein protects T-cells from IL2 deprivation-induced apoptosis by inhibiting FOXO3A transcriptional activity, leading to down-regulation of the pro-apoptotic factor BCL2L11.
  Related references:
  PMID:15031210
• In macrophages, TSC22D3 mediates the anti-inflammatory and immunosuppressive effects of glucocorticoids and IL10.
  Related references:
  PMID:12393603
• In T-cells, it inhibits anti-CD3-induced NF-kB nuclear translocation and DNA-binding activity.
  Related references:
  PMID:11468175
• TSC22D3 suppresses AP-1 transcription factor complex DNA-binding activities in vitro (By similarity).
• The protein inhibits myogenic differentiation and mediates anti-myogenic effects of glucocorticoids by binding and regulating MYOD1 and HDAC1 transcriptional activity.
• Subcellular localization is both cytoplasmic and nuclear.
• TSC22D3 is ubiquitously expressed, with highest levels in brain, lung, spleen, and skeletal muscle, lower levels in heart and kidney, and no expression in pancreas. The major source of constitutive expression is the macrophage lineage.
  Related references:
  PMID:11313722
  PMID:12393603
• It is down-regulated in activated macrophages from inflammatory lesions such as tuberculosis and Crohn’s disease, but persists in macrophages phagocytosing apoptotic cells in Burkitt lymphoma.
  Related references:
  PMID:12393603

Experimental Guidance and Technical Tips

• The immunogen corresponds to an N-terminal peptide (amino acids 1-100); the antibody may detect multiple isoforms or cleavage products. Consider confirming specificity using appropriate positive and negative controls.
• For immunofluorescence/immunocytochemistry (IF/ICC), optimize fixation and permeabilization conditions for your cell type, as TSC22D3 is expressed in both cytoplasm and nucleus.
• ELISA can be used to quantify TSC22D3 levels; ensure the antibody's reactivity with native and recombinant protein is validated in your sample matrix.
• Cross-reactivity with rat suggests potential use in rodent models; however, always validate species-specific detection in your experimental system.
• Given TSC22D3's role in glucocorticoid signaling, consider using glucocorticoid-treated samples as positive controls to confirm detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive raw material sourcing for IVD developers and researchers focusing on glucocorticoid signaling, apoptosis, and inflammation. From validated antibody pairs and bulk ancillary reagents to rare target sourcing for TSC22D3 and related proteins, we support your assay development from concept to clinic.