Anti-TSC2 (Tuberin) Rabbit Monoclonal Antibody for WB, IP, ELISA - P49815

Core Product Specifications and Parameters

Biological Background: Tuberin Function and Localization

• Tuberin (also known as TSC2) is the protein product of the TSC2 gene; aliases include LAM, TSC4, and PPP1R160.
• It serves as the catalytic component of the TSC-TBC complex, functioning as a GTPase-activating protein (GAP) for RHEB, a direct activator of mTORC1. Related references: PMID:12172553 PMID:12820960 PMID:12842888
• Under nutrient-deprived conditions, the TSC-TBC complex inhibits mTORC1, blocking phosphorylation of downstream effectors like RPS6KB1 and EIF4EBP1. Related references: PMID:12172553 PMID:12271141 PMID:12842888
• Nutrient signals inactivate the TSC-TBC complex, relieving mTORC1 suppression to promote anabolic cell growth. Related references: PMID:12172553 PMID:24529379
• Subcellularly, tuberin localizes to the lysosome membrane and the cytosol.
• It is broadly expressed, with notable levels in liver, brain, heart, lymphocytes, fibroblasts, biliary epithelium, pancreas, skeletal muscle, kidney, lung, and placenta.
• Tuberin is regulated by phosphorylation (a phosphoprotein) and ubiquitin conjugation (Ubl conjugation); mutations in TSC2 are linked to tuberous sclerosis complex, and the protein acts as a tumor suppressor.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1700–1807 of human tuberin (P49815); antibody recognition may be epitope‑specific and may not cross‑react with other species unless experimentally verified.
• For Western blot, the full‑length tuberin has a predicted molecular mass of ~201 kDa; use a low‑percentage SDS‑PAGE gel (e.g., 6%) with extended transfer times and a suitable high‑molecular‑weight marker.
• For immunoprecipitation, validate enrichment of tuberin complexes in the relevant cell lysate; include appropriate negative controls.
• ELISA performance can be assessed using the peptide immunogen as a coating antigen, though reactivity against the native protein should be confirmed in the specific assay format.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with a reliable supply of IVD antibody raw materials, including this tuberin‑specific clone for mTOR/TSC pathway studies. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and support for rare‑target sourcing to streamline your development pipeline.