Anti-TSC2 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P49815

Core Product Specifications and Parameters

Biological Background: TSC2 Function and Localization

• Tuberin (TSC2) is the protein product of the TSC2 gene, a tumor suppressor that forms a complex with TSC1 (hamartin) to constitute the TSC-TBC complex.
• TSC2 functions as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of mTORC1 kinase activity (PubMed:12172553, PubMed:12820960, PubMed:12842888). Related references: PMID:12172553, PMID:12820960, PMID:12842888
• Under nutrient-deprived conditions, the TSC-TBC complex inhibits mTORC1, preventing phosphorylation of downstream effectors such as ribosomal protein S6 kinase and 4E-BP1 (PubMed:12172553, PubMed:12271141, PubMed:12842888). Related references: PMID:12172553, PMID:12271141, PMID:12842888
• In response to growth factors and amino acids, the TSC-TBC complex is inactivated, relieving inhibition of mTORC1 and promoting anabolic cell growth (PubMed:12172553, PubMed:24529379). Related references: PMID:12172553, PMID:24529379
• TSC2 localizes to lysosomal membranes and the cytosol, where it regulates RHEB and mTORC1 signaling.
• TSC2 is widely expressed in tissues including liver, brain, heart, kidney, and skeletal muscle, consistent with its fundamental role in nutrient sensing and growth control.
• Mutations in TSC2 cause tuberous sclerosis complex, characterized by multi-organ benign tumors and neurological manifestations such as epilepsy.
• The protein undergoes post-translational modifications such as phosphorylation and ubiquitination, which modulate its activity and stability.

Experimental Guidance and Technical Tips

• For western blotting, TSC2 is a large protein (~201 kDa); consider using a low-percentage (e.g., 6–8%) polyacrylamide gel and longer transfer times to ensure efficient membrane transfer.
• In immunohistochemistry (IHC-P), optimize antigen retrieval and validate staining in tissues with known high TSC2 expression, such as kidney or brain.
• For immunofluorescence (IF/ICC), confirm TSC2 subcellular localization (cytoplasmic/lysosomal) in your cell lines of interest.
• When using this polyclonal antibody for ELISA, pair it with a suitable detection antibody and validate against recombinant TSC2 or cell lysates.
• As a polyclonal antibody, lot-to-lot consistency should be verified for critical experiments; consider performing a standard curve with known positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement for diagnostic manufacturers and research laboratories, offering one‑stop access to validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare‑target sourcing. For TSC2 and mTOR‑pathway studies, we supply high‑quality antibodies and complementary research reagents to accelerate your development from concept to clinic. Contact us to discuss your specific TSC2 project requirements and custom sourcing needs.