Anti-TSC2 Rabbit Polyclonal Antibody for IF/ICC, ELISA - P49815

Core Product Specifications and Parameters

Biological Background: TSC2 Function and Localization

• Tuberin (TSC2) acts as the catalytic component of the TSC-TBC complex, a key negative regulator of the mTORC1 signaling pathway, which controls cell growth and metabolism.
  Related references: PMID:12172553, PMID:12271141, PMID:12842888
• Within the complex, TSC2 functions as a GTPase-activating protein (GAP) for the small GTPase RHEB, thereby inhibiting mTORC1 kinase activity.
  Related references: PMID:12172553, PMID:12820960, PMID:12842888
• The TSC-TBC complex is regulated by nutrient availability: under nutrient deprivation, it suppresses mTORC1, while nutrient abundance inactivates the complex to allow mTORC1 signaling.
  Related references: PMID:12172553, PMID:24529379
• TSC2 also stimulates the intrinsic GTPase activity of RAP1A and RAB5, implicating it in additional signaling pathways.
• Tuberin localizes primarily to the lysosome membrane and cytosol, consistent with its role in nutrient sensing.
• It is widely expressed in tissues including liver, brain, heart, lymphocytes, fibroblasts, biliary epithelium, pancreas, skeletal muscle, kidney, lung, and placenta.
• Mutations in TSC2 are associated with tuberous sclerosis complex, epilepsy, and tumor suppression; the protein undergoes phosphorylation and ubiquitin conjugation.

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal region (aa 1531-1807), which contains the GAP domain; consider this when assessing isoform specificity.
• For immunofluorescence (IF/ICC), use fixation and permeabilization conditions appropriate for cytosolic and membrane-associated antigens; validate in relevant cell lines (e.g., HeLa, HEK293).
• In ELISA applications, optimize coating conditions and antibody concentrations; pairing with a capture antibody specific for TSC2 may enhance detection.
• Cross-reactivity with mouse and rat is anticipated, but verify performance in your specific sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to a comprehensive portfolio of IVD raw materials, including validated antibodies, recombinant proteins, and ancillary reagents. This anti-TSC2 polyclonal antibody supports investigations into mTORC1 signaling, nutrient sensing, and associated pathologies such as tuberous sclerosis and cancer. Leverage CamelBio’s one-stop sourcing capabilities for bulk reagents, custom antibody development, and rare target materials to accelerate your assay development pipeline.