Anti-Tryptophanyl-tRNA synthetase 1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P23381

Core Product Specifications and Parameters

Biological Background: Tryptophanyl-tRNA synthetase 1 Function and Localization

• Tryptophanyl-tRNA synthetase 1 (WARS1), also known as interferon-induced protein 53 (IFP53) or hWRS, is encoded by the WARS1 gene. Alternative splicing generates multiple isoforms with distinct functional properties.
• The full-length enzyme catalyzes tryptophan activation by ATP to form Trp-AMP and its subsequent transfer to tRNA(Trp), a critical step in cytoplasmic protein biosynthesis.
• Shorter isoforms, such as IFP53, lack aminoacylation activity but exert angiostatic effects by inhibiting fluid shear stress-activated responses in endothelial cells. They regulate ERK, Akt, and eNOS pathways linked to angiogenesis and cytoskeletal reorganization. Related references: PMID:11773625, PMID:11773626, PMID:14630953
• The protein is primarily localized in the cytoplasm and is subject to phosphorylation, which may modulate its activity.
• Interferon gamma induces WARS1 expression, linking it to immune responses and cellular stress pathways.
• Disease-associated keywords include intellectual disability, neurodegeneration, and neuropathy, highlighting potential clinical relevance.

Experimental Guidance and Technical Tips

• For Western blotting, a band near 53 kDa is expected; optimize blocking and antibody dilution using relevant cell or tissue lysates.
• In immunohistochemistry (paraffin-embedded sections), antigen retrieval may be required; validate with appropriate positive and negative controls.
• ELISA applications can leverage the peptide immunogen (aa 372–471) as a capture or detection reagent; cross-reactivity with other family members should be assessed.
• The immunogen’s C-terminal position may allow recognition of both full-length and truncated isoforms; confirm isoform specificity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, including validated antibody pairs and bulk ancillary reagents, to support diagnostics and life science research. For Tryptophanyl-tRNA synthetase 1, a key enzyme in protein synthesis and angiogenesis, we offer optimized rabbit monoclonal antibodies to accelerate your assay development. Our end-to-end sourcing services cover every stage from concept to clinic, ensuring reliable access to rare and established targets.