Anti-Tryptophanyl-tRNA synthetase 1 Polyclonal Antibody for WB, ELISA - P23381

Core Product Specifications and Parameters

Biological Background: Tryptophanyl-tRNA synthetase 1 Function and Localization

• Tryptophanyl-tRNA synthetase 1, also designated tryptophan--tRNA ligase, cytoplasmic, is encoded by the WARS1 gene and commonly referred to as TrpRS, IFP53, or WARS.
• It catalyzes the ATP-dependent ligation of tryptophan to its cognate tRNA, a critical step in cytoplasmic protein synthesis.
• Alternative splicing generates isoforms with distinct functions; one isoform lacks tRNA aminoacylation activity but acts as an angiostatic factor by inhibiting endothelial cell responses to fluid shear stress and modulating ERK/Akt/eNOS signaling pathways. Related references: PMID:11773625 / PMID:11773626 / PMID:14630953
• The protein localizes primarily within the cytoplasm.
• It belongs to the class-I aminoacyl-tRNA synthetase family and contains conserved ATP-binding and nucleotide-binding motifs.
• Post-translational modifications include phosphorylation, and the protein is implicated in neurological disorders such as neurodegeneration and neuropathy.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (amino acids 1-270) of human TrpRS. For Western blot, expect a band near 53 kDa under reducing conditions; multiple bands may appear due to alternative splicing or post-translational modifications.
• In ELISA applications, consider that the polyclonal nature may capture both full-length and angiostatic isoforms; validate specificity with recombinant isoform controls.
• When using mouse or rat samples, verify cross-reactivity in the target tissue and consider that sequence homology in the immunogen region may influence detection efficiency.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable access to IVD raw materials, from concept to clinical application. This anti-TrpRS polyclonal antibody supports translational and angiogenesis research, and we offer a full portfolio of validated antibody pairs, optimized monoclonal and polyclonal reagents, bulk ancillary materials, and tailored sourcing for rare targets. Partner with CamelBio to streamline your raw-material procurement and accelerate assay development.