Anti-Tryptophanyl-tRNA synthetase 1 Monoclonal Antibody for WB & ELISA - P23381

Core Product Specifications and Parameters

Biological Background: Tryptophanyl-tRNA synthetase 1 (WARS1) Function and Localization

• Tryptophanyl-tRNA synthetase 1 (WARS1; also known as TrpRS, IFP53, IFI53) is a member of the class-I aminoacyl-tRNA synthetase family and is essential for charging tRNA(Trp) with tryptophan.
• The canonical full-length enzyme localizes to the cytoplasm and catalyzes the two-step aminoacylation reaction: tryptophan activation by ATP followed by transfer to tRNA(Trp), a fundamental process in translation.
• Alternative splicing generates a truncated isoform that lacks tRNA synthetase activity but instead functions as an angiostatic factor, inhibiting endothelial cell responses (PubMed:11773625, PubMed:11773626). Related references: PMID:11773625 PMID:11773626
• This angiostatic isoform downregulates fluid shear stress-induced signaling, suppressing ERK, Akt, and eNOS activation pathways involved in angiogenesis and cytoskeletal reorganization (PubMed:14630953). Related references: PMID:14630953
• WARS1 is also an interferon-inducible protein (IFI53), suggesting roles in immune modulation and host defense.
• Post-translational modifications, including phosphorylation, along with ATP- and nucleotide-binding domains, regulate its enzymatic and signaling functions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 3-98) of human WARS1, which may recognize both full-length and truncated isoforms; consider validating isoform specificity in your system.
• For Western blotting, a band around 53 kDa is expected in human, mouse, and rat lysates; optimize the antibody dilution based on your sample and detection protocol.
• In ELISA applications, this antibody may detect recombinant or endogenous WARS1; include appropriate controls to confirm target specificity.
• Cross-reactivity with mouse and rat suggests conserved epitopes, but verify performance if using tissues or cells from other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-Tryptophanyl-tRNA synthetase 1 monoclonal antibody is well-suited for investigations into protein translation and angiogenesis. We provide end-to-end technical and procurement support to accelerate your diagnostic development from concept to clinic.