Anti-Trypsin Rabbit Monoclonal Antibody for IF-P, IHC-P, ELISA - P07477

Core Product Specifications and Parameters

Biological Background: Trypsin Function and Localization

• Trypsin, encoded by the PRSS1 gene, is also known as serine protease 1 (aliases: TRP1, TRY1, TRY4, TRYP1) and functions as a key digestive enzyme.
• It is secreted into the extracellular space and plays a central role in protein digestion by cleaving peptide bonds at arginine or lysine residues.
• As a serine protease, it exhibits hydrolase activity against synthetic substrates including Boc-Phe-Ser-Arg-Mec, Boc-Leu-Thr-Arg-Mec, Boc-Gln-Ala-Arg-Mec, and Boc-Val-Pro-Arg-Mec.
• Trypsin is initially produced as the inactive zymogen trypsinogen and undergoes proteolytic activation; the single-chain form may be more active than the two-chain form on certain substrates.
• The protein contains calcium-binding sites, disulfide bonds, and metal-binding motifs, and its structure has been determined (3D-structure).
• Keywords: 3D-structure, Calcium, Digestion, Direct protein sequencing, Disease variant, Disulfide bond, Hydrolase, Metal-binding, Protease, Proteomics identification, Reference proteome, Secreted, Serine protease, Signal, Sulfation, Zymogen.
• Subcellular localization is extracellular (secreted), in line with its digestive function.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 24–247, covering the mature trypsinogen region; the antibody is expected to recognize both zymogen and activated trypsin forms.
• For IHC-P, consider standard antigen retrieval protocols and validate with appropriate tissue controls (e.g., pancreas) to ensure specific staining.
• In ELISA applications, pairing with a detection antibody may enable sandwich assay development; cross-reactivity with other trypsin isoforms should be assessed if sample complexity is high.
• Use protease inhibitor cocktails when preparing biological samples to prevent degradation of the target or antibody.
• The antibody has been tested in IF-P, IHC-P, and ELISA; optimal dilutions and conditions should be determined empirically by the end user.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutes with a comprehensive supply of IVD raw materials, including validated antibody pairs and optimized monoclonal/polyclonal antibodies. This Trypsin Rabbit mAb is relevant for digestive enzyme and protease studies. Beyond individual reagents, CamelBio offers bulk ancillary reagents and facilitates rare-target raw-material sourcing to streamline your assay development from concept to clinic.