Anti-TRβ1/THRB Rabbit Monoclonal Antibody for WB and ELISA - P10828

Core Product Specifications and Parameters

Biological Background: TRβ1/THRB Function and Localization

• TRβ1/THRB is a nuclear hormone receptor that functions as a ligand-dependent transcription factor, acting as either a repressor or activator of transcription.
• It serves as a high-affinity receptor for thyroid hormones, including triiodothyronine (T3) and thyroxine (T4), mediating their cellular effects.
• The protein belongs to the nuclear receptor subfamily 1 group A member 2 (NR1A2), and is also known as c-erbA-2 or c-erbA-beta.
• Multiple isoforms are produced through alternative splicing (THRB1, THRB2), which may exhibit distinct tissue-specific expression and functional roles.
• TRβ1/THRB localizes to the nucleus, consistent with its role as a DNA-binding transcription factor.
• It contains conserved DNA-binding domains with zinc-finger motifs, enabling recognition of thyroid hormone response elements (TREs) in target gene promoters.
• Keywords such as "Deafness" and "Disease variant" indicate that mutations in THRB are associated with resistance to thyroid hormone and hearing loss.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-91 of human TRβ1/THRB (NP_000452.2), a region in the N-terminal domain. This may allow recognition of specific isoforms or provide distinct epitope accessibility.
• For Western blotting, the predicted molecular weight is approximately 53 kDa. Use appropriate loading controls and validate the antibody in your specific sample system, as post-translational modifications may shift the observed band.
• In ELISA applications, consider using recombinant TRβ1 protein or cell lysates expressing the target. Ensure proper blocking and washing conditions to minimize background.
• As a rabbit monoclonal antibody, expect high specificity and lot-to-lot consistency; however, validate performance under your experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD manufacturers and researchers, offering one-stop access to validated antibodies, bulk ancillary reagents, and rare raw materials. For thyroid hormone receptor studies, we can support your development needs with optimized antibody pairs and customized reagents. Our supply chain is designed to accelerate your transition from concept to clinic.