Anti-TRPV5 Polyclonal Antibody (Rabbit) for WB, IHC-P, ELISA - Q9NQA5

Core Product Specifications and Parameters

Biological Background: TRPV5 Function and Localization

• TRPV5 (Transient receptor potential cation channel subfamily V member 5) is also known as calcium transport protein 2 (CaT2), epithelial calcium channel 1 (ECaC1), and osm-9-like TRP channel 3 (OTRPC3).
• It is a constitutively active, calcium‑selective cation channel essential for Ca²⁺ reabsorption in the kidney and intestine. Related references: PMID:11549322 PMID:18768590
• Channel activation occurs under low intracellular Ca²⁺, with the current showing inward rectification. Related references: PMID:11549322 PMID:18768590
• Ca²⁺‑dependent feedback regulation involves fast channel inactivation and slow current decay (By similarity).
• Heteromeric assembly with TRPV6 alters channel properties, including conferring voltage‑dependent gating (By similarity).
• Subcellular localization is at the apical cell membrane.
• Highly expressed in kidney, small intestine, and pancreas; lower expression in testis, prostate, placenta, brain, colon, and rectum.
• Post‑translational modifications include glycosylation and phosphorylation; the protein contains ANK repeats and a calmodulin‑binding domain.

Experimental Guidance and Technical Tips

• For Western blot (WB), the predicted band size is approximately 83 kDa; a band near this molecular weight should be observed in positive control lysates.
• For immunohistochemistry (IHC‑P), antigen retrieval and antibody titration are recommended; validate using kidney or intestinal tissue sections as positive controls.
• For ELISA, optimize coating antigen concentration and antibody dilution for your specific assay format.
• The antibody cross‑reacts with human, mouse, and rat; it may be suitable for studies in these species, but independent validation is advised.
• As a polyclonal antibody, lot‑to‑lot consistency should be assessed, and appropriate species‑matched secondary antibodies should be used.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with comprehensive IVD raw material sourcing, from concept to clinic. This anti‑TRPV5 polyclonal antibody is part of our portfolio for calcium channel and kidney function research. In addition to this product, we offer validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare‑target raw materials to accelerate your IVD development.