Anti-TRPV4 Polyclonal Antibody for WB and ELISA - Q9HBA0

Core Product Specifications and Parameters

Biological Background: TRPV4 Function and Localization

• TRPV4 is a member of the transient receptor potential (TRP) channel family, encoded by the TRPV4 gene, also designated as VRL-2, OTRPC4, and VR-OAC.
• TRPV4 functions as a non-selective calcium-permeant cation channel activated by osmotic and mechanical stimuli, heat, low pH, citrate, and phorbol esters. Related references: PMID:16293632 PMID:18695040 PMID:18826956 PMID:29899501
• Channel activity is regulated by a calmodulin-dependent negative feedback mechanism and intracellular Ca(2+) levels. Related references: PMID:12724311 PMID:18826956
• TRPV4 localizes to the cell membrane, apical membrane, adherens junctions, primary cilia, and the endoplasmic reticulum.
• At the protein level, TRPV4 is found in synoviocytes from rheumatoid arthritis patients and healthy controls. Related references: PMID:19759329
• TRPV4 is required for normal development and maintenance of bone and cartilage. Related references: PMID:26249260
• Mutations in TRPV4 are associated with skeletal dysplasias, Charcot-Marie-Tooth disease, and other neuropathies.
• TRPV4 facilitates replication of hepatitis C virus, Dengue virus, and Zika virus, likely through interaction with DDX3X.

Experimental Guidance and Technical Tips

• The immunogen—a synthetic peptide spanning residues 800–871 within the C‑terminal intracellular domain of human TRPV4—was designed to target a region unique to this channel, minimizing cross‑reactivity with other TRP family members.
• For Western blotting (WB), the predicted molecular weight of human TRPV4 is 98 kDa; consider optimizing protein extraction and gel conditions to resolve this band clearly.
• In ELISA applications, pairing this antibody with a matched detection reagent may enhance assay sensitivity; validate the system using recombinant TRPV4 or peptide‑coated plates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a streamlined, one‑stop channel for IVD raw‑material sourcing, technical services, and consulting support. This TRPV4 rabbit polyclonal antibody, suitable for WB and ELISA, is one component of our extensive catalogue of validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare‑target raw materials designed to accelerate your assay development. With expertise spanning the entire IVD pipeline—from concept to clinic—CamelBio helps you secure reliable, high‑quality immunoreagents for ion channel and mechanobiology research.