Anti-TRPV4 Monoclonal Antibody for WB & ELISA - Q9HBA0

Core Product Specifications and Parameters

Biological Background: TRPV4 Function and Localization

• TRPV4 (Transient receptor potential cation channel subfamily V member 4) is a non-selective calcium-permeant cation channel activated by hypotonicity, heat, low pH, citrate, and phorbol esters, triggering calcium influx with outward rectification. Related references: PMID:16293632 PMID:18695040 PMID:18826956 PMID:29899501
• It functions as a molecular sensor for osmotic and mechanical stimuli, and together with PKD2, forms mechano- and thermosensitive channels in primary cilia. Related references: PMID:18695040 PMID:18826956 PMID:29899501
• In synoviocytes, TRPV4 mediates intracellular calcium elevation induced by 4α-phorbol 12,13-didecanoate and hypotonic stimulation, and regulates pro-inflammatory IL-8 production. Related references: PMID:19759329
• TRPV4 is required for normal development and maintenance of bone and cartilage; mutations in TRPV4 are linked to skeletal dysplasias and Charcot-Marie-Tooth disease. Related references: PMID:26249260
• Subcellularly, TRPV4 localizes to the plasma membrane, apical membrane, adherens junctions, primary cilia, and the endoplasmic reticulum.
• The channel modulates cell-cell junction integrity in keratinocytes and participates in regulatory volume decrease in salivary epithelial cells together with AQP5.
• In the central nervous system, TRPV4 may trigger voluntary water intake in response to hypernatremia.
• Additionally, TRPV4 facilitates replication of Hepatitis C, Dengue, and Zika viruses, likely through its interaction with DDX3X.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal peptide (amino acids 771-871) of human TRPV4; when testing new sample types, validate specificity using positive and negative controls.
• For Western blotting, a predominant band near 98 kDa is expected; optimize blocking and antibody dilution based on the sample system.
• In ELISA applications, consider using recombinant TRPV4 protein or cell lysates from TRPV4-expressing cells to confirm reactivity.
• Cross-reactivity with other TRPV family members is not expected, but independent verification is recommended if studying related channels.

CamelBio: Your One-Stop Sourcing Bridge

As a comprehensive IVD raw material partner, CamelBio supplies this high-quality anti-TRPV4 monoclonal antibody alongside a full spectrum of ancillary reagents—including validated antibody pairs, optimized recombinant proteins, and bulk buffer solutions—to streamline your calcium channel, mechanobiology, or bone disease research and assay development. Our sourcing capabilities extend to rare target reagents and custom immunogen design, helping you bridge from concept to clinical application efficiently. Explore our catalog for one-stop procurement of all your IVD raw material needs for TRPV4-related projects.