Anti-TRPV1 Rabbit Monoclonal Antibody for WB, ELISA - Q8NER1

Core Product Specifications and Parameters

Biological Background: TRPV1 Function and Localization

• TRPV1 is a non-selective calcium-permeant cation channel that detects noxious chemical and thermal stimuli, and mediates proton influx, contributing to inflammatory pain and hyperalgesia. Related references: PMID:11050376 PMID:11243859 PMID:11226139
• It is directly activated by vanilloids such as capsaicin and by temperatures exceeding 42°C. Related references: PMID:37117175
• The channel also acts as an ionotropic endocannabinoid receptor, triggering a form of long-term depression in the hippocampus and nucleus accumbens.
• TRPV1 localizes to the postsynaptic cell membrane and dendritic spine membranes.
• It is widely expressed at low levels, with elevated expression in dorsal root ganglia; in skin, it is found in sensory nerve fibers, keratinocytes, and sweat glands.
• The protein undergoes alternative splicing and phosphorylation, and contains multiple transmembrane helices and ankyrin repeats.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 725-839), which may detect total TRPV1 or specific isoforms; users should validate isoform specificity in their experimental system.
• For Western blotting, a band near 95kDa is expected; consider using appropriate membrane and detection conditions for this size.
• For ELISA applications, validate the antibody with the recombinant protein or relevant cell lysates under optimal coating conditions.
• Cross-reactivity with mouse and rat TRPV1 is indicated; verify performance with appropriate tissue or cell lysates from these species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with a comprehensive portfolio of IVD raw materials, including validated monoclonal antibodies, matched pairs, and bulk ancillary reagents. For targets like TRPV1—critical in pain and sensory neuroscience—our one-stop sourcing capability streamlines assay development from concept to clinic. Contact us for tailored procurement solutions to secure the raw materials your project demands.