Anti-TRPS1 Rabbit Polyclonal Antibody for WB, ELISA - Q9UHF7

Core Product Specifications and Parameters

Biological Background: TRPS1 Function and Localization

• Zinc finger transcription factor Trps1, also known as Tricho-rhino-phalangeal syndrome type I protein or GC79, is a member of the GATA-binding family of transcriptional repressors.
• It functions as a transcriptional repressor by binding to GATA sequences, regulating the expression of GATA-controlled genes during vertebrate development.
• TRPS1 plays a critical role in chondrocyte proliferation and differentiation, expanding distal chondrocytes, activating proliferation in columnar cells, and supporting hypertrophic differentiation.
• The protein localizes to the nucleus, consistent with its role in transcriptional regulation.
• It is ubiquitously expressed in adult tissues and found in fetal brain, lung, kidney, liver, spleen, and thymus; expression levels differ between androgen-dependent and androgen-independent prostate cancer cells.
• TRPS1 undergoes alternative splicing and is subject to post-translational modifications including phosphorylation and ubiquitination. It contains multiple zinc-finger domains for DNA binding.
• Mutations in TRPS1 are associated with tricho-rhino-phalangeal syndrome, highlighting its importance in craniofacial and skeletal development.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 20–260 of human TRPS1, a region within the N-terminal portion of the protein. Consider this epitope location when designing downstream assays.
• For Western blot, the predicted molecular weight is approximately 142 kDa; validate band specificity using appropriate positive and negative controls.
• This polyclonal antibody is produced in rabbit and has been shown to cross-react with mouse and rat TRPS1 according to supplier specifications, making it suitable for comparative studies across these species.
• For ELISA, consider pairing with a suitable detection system and validate performance in your specific sample matrix to ensure sensitivity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes streamlined access to high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our capabilities cover a broad range of targets—from common biomarkers to rare transcriptional regulators like TRPS1—supported by technical consultation from concept to clinical application. Partner with CamelBio to efficiently source the critical materials needed for your developmental biology and skeletal disease research.