Anti-TRPM8 Rabbit Polyclonal Antibody for WB, ELISA - Q7Z2W7

Core Product Specifications and Parameters

Biological Background: TRPM8 Function and Localization

• TRPM8 is a non-selective cation channel permeable to monovalent and divalent cations, with higher permeability for Ca(2+). It is activated by cool temperatures (<23-28°C) and chemical ligands such as menthol and icilin, and serves as a primary cold sensor in peripheral sensory neurons. Related references: PMID:15306801, PMID:15852009, PMID:16174775
• The channel features voltage-dependent gating; cooling or chemical agonists shift its activation threshold toward physiological membrane potentials, enabling temperature-dependent depolar. Related references: PMID:15306801
• Beyond thermosensation, TRPM8 regulates basal tear secretion by sensing evaporation-induced cooling and changes in osmolality at the corneal surface.
• In prostate cancer, TRPM8 may contribute to cell migration and invasion, and its expression is elevated in prostate tumors and other malignancies including colon, lung, breast, and skin tumors. Related references: PMID:16174775, PMID:25559186
• TRPM8 localizes to the plasma membrane, membrane rafts, and the endoplasmic reticulum membrane, consistent with roles in rapid sensory signaling and intracellular calcium handling.
• The protein is a member of the transient receptor potential (TRP) channel family and contains six transmembrane domains, coiled-coil regions, and a TRP domain, characteristic of TRPM subfamily members.
• Post-translational modifications include glycosylation, and the channel activity may be modulated by alternative splicing to yield isoforms with distinct regulatory properties.

Experimental Guidance and Technical Tips

• The immunogen encompasses the cytosolic C-terminus (aa 980-1104), a region likely accessible in denaturing Western blot; a band around 128 kDa is expected based on the theoretical molecular weight.
• For Western blot, use standard SDS-PAGE and transfer to PVDF or nitrocellulose; optimize blocking and detection conditions for this moderate-to-large membrane protein.
• ELISA applications may use direct or indirect antigen coating; titrate antibody to determine optimal working concentration for the assay format.
• Validate antibody specificity in relevant human cell lines or tissues known to express TRPM8, such as prostate cancer lines (e.g., LNCaP) or sensory neuron models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a seamless procurement experience for IVD raw materials, supporting research and development from concept to clinic. For TRPM8-focused studies in sensory biology or oncology, we provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare targets. Our integrated supply chain ensures reliable access to the critical tools you need to advance your diagnostic assays.