Anti-TRPM8 Rabbit Polyclonal Antibody for WB, ELISA - Q7Z2W7

Core Product Specifications and Parameters

Biological Background: TRPM8 Function and Localization

• TRPM8 is a non-selective cation channel permeable to Na+, K+, and Ca2+ (with higher Ca2+ permeability), activated by cool temperatures (<23-28°C) and chemical ligands like menthol and icilin. Related references: PMID:15306801 PMID:15852009 PMID:16174775
• It functions as a voltage-dependent channel; activation by cold or menthol shifts voltage thresholds toward physiological potentials, playing a central role in cold thermosensation. Related references: PMID:15306801
• In addition to thermosensation, TRPM8 regulates basal tear secretion by sensing evaporation-induced cooling and osmolality changes.
• TRPM8 activity is negatively regulated by alternative isoforms that stabilize the closed state.
• The protein localizes to the cell membrane, membrane rafts, and endoplasmic reticulum membrane.
• It is expressed in the prostate and in various primary tumors including colon, lung, breast, and skin tumors.
• TRPM8 may contribute to prostate cancer cell migration. Related references: PMID:16174775 PMID:25559186
• The channel is a glycoprotein with multiple transmembrane helices and a coiled-coil domain, and it contains calcium-binding sites.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 880–960 of human TRPM8, a region that may be conserved across isoforms; verify specificity in your experimental system.
• For Western blot, the predicted molecular weight is 128 kDa; use appropriate gel percentage (e.g., 8% gel) and extended transfer times for high-molecular-weight proteins.
• For ELISA, validate the antibody with human and mouse samples and consider potential cross-reactivity with other TRPM family members.
• As a polyclonal antibody, monitor lot-to-lot consistency for reproducibility.
• TRPM8 is a multi-pass membrane protein; ensure efficient protein extraction and include relevant loading controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—from concept to clinic. This anti-TRPM8 antibody supports research in cold sensing, ion channel biology, and cancer, and is part of our catalog of optimized polyclonal antibodies. We also offer validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets to streamline your development pipeline.