Anti-TRPM8 Rabbit Monoclonal Antibody for WB, ELISA - Q7Z2W7

Core Product Specifications and Parameters

Biological Background: TRPM8 Function and Localization

• TRPM8 (Transient Receptor Potential Cation Channel Subfamily M Member 8), also known as LTrpC-6 and Trp-p8, is a transmembrane ion channel of the TRPM subfamily.
• It functions as a cold sensor, activated by cool temperatures (<23–28°C) and chemical ligands such as menthol and icilin, leading to non-selective cation influx (Na⁺, K⁺, Ca²⁺) with higher Ca²⁺ permeability. Related references: PMID:15306801 PMID:15852009 PMID:16174775
• Channel activation by cold or ligands shifts the voltage dependence of TRPM8 towards physiological membrane potentials, facilitating opening. Related references: PMID:15306801
• TRPM8 may contribute to prostate cancer cell migration, suggesting a role in tumor biology. Related references: PMID:16174775 PMID:25559186
• A regulatory mechanism negatively modulates menthol- and cold-induced channel activity by stabilizing the closed state of the channel.
• The protein localizes to the cell membrane, membrane rafts, and endoplasmic reticulum membrane, consistent with its ion channel and signaling functions.
• TRPM8 is primarily expressed in prostate tissue and is also found in prostate tumors and other primary tumors (colon, lung, breast, skin), highlighting its potential as a diagnostic or therapeutic target.

Experimental Guidance and Technical Tips

• The immunogen (synthetic peptide from the cytoplasmic region, aa 900–1000) is expected to recognize the native protein; however, experimental validation in the relevant sample system is advised.
• For Western blot, due to the large predicted size (128 kDa), consider using a gradient gel and optimized transfer conditions for high-molecular-weight proteins.
• When performing ELISA, titrate the antibody and include appropriate positive and negative controls to confirm specific binding in your assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with streamlined access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. Whether you are developing assays for ion channel research, oncology biomarkers, or other specialized applications, our supply network supports your project from concept to clinic. Leverage our expertise to secure high-quality TRPM8 reagents efficiently.