Anti-TRPM4 Polyclonal Antibody for WB, ELISA - Q8TD43

Core Product Specifications and Parameters

Biological Background: TRPM4 Function and Localization

• TRPM4, also known as melastatin-4 or LTrpC4, is a member of the transient receptor potential (TRP) channel family and functions as a calcium-activated non-selective cation channel.
• It mediates membrane depolarization by conducting monovalent cations (relative permeability: Na+ > K+ > Cs+ > Li+), while being impermeable to Ca2+ itself. Related references: PMID:12015988 PMID:12842017 PMID:29211723
• TRPM4 plays essential roles in diverse tissues: it contributes to pacemaking activity in cardiomyocytes, insulin secretion in pancreatic beta-cells, myogenic constriction of cerebral arteries, and neuronal function in entorhinal cortex and sensory neurons.
• In the immune system, it regulates T-cell activation by modulating Ca2+ oscillations required for NFAT-dependent IL2 production, and differentially controls T-helper 1 (Th1) and Th2 cell motility and cytokine production.
• TRPM4 enhances cell proliferation through up-regulation of the beta-catenin signaling pathway and is involved in keratinocyte differentiation. Related references: PMID:30528822
• The protein localizes to the cell membrane, endoplasmic reticulum, and Golgi apparatus, and is subject to post-translational modifications including glycosylation, phosphorylation, and ubiquitination.
• Widely expressed with highest levels in intestine and prostate, it is also prominently found in Purkinje fibers of the heart, Th2 cells, and keratocytes.
• Mutations in TRPM4 are associated with progressive familial heart block type IB (PFHB1B) and palmoplantar keratoderma, underscoring its importance in cardiac conduction and skin homeostasis.
• Alternative splicing produces isoforms; some variants may lack channel activity.

Experimental Guidance and Technical Tips

• The immunogen is the N-terminal region (aa 1-240) of human TRPM4, which may detect multiple splice isoforms.
• For Western blot, expect a band near 134 kDa; validate with positive controls from human, mouse, or rat tissues known to express TRPM4.
• In ELISA, test antibody dilution series and optimize coating antigen concentration to achieve the desired sensitivity.
• Pre-adsorption or blocking peptides can be used to confirm band specificity in WB.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials. This anti-TRPM4 polyclonal antibody supports investigations into cardiac electrophysiology, ion channel disorders, and immune cell signaling. Beyond individual antibodies, our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for challenging targets, enabling a seamless transition from concept to clinical application.