Anti-TRPM2 Rabbit Polyclonal Antibody for WB, IF-P, ELISA - O94759

Core Product Specifications and Parameters

Biological Background: TRPM2 Function and Localization

• TRPM2 (Transient receptor potential cation channel subfamily M member 2) is a nonselective, voltage-independent cation channel that mediates Na+ and Ca2+ influx, thereby raising cytoplasmic Ca2+ levels. Related references: PMID:11385575 PMID:11509734 PMID:11804595

• Channel gating is activated by intracellular ADP-ribose, Ca2+, warm temperature, and oxidative stress, with ADP-ribose binding to the Nudix domain inducing a conformational change that primes the channel for Ca2+-dependent opening. Related references: PMID:19171771 PMID:25620041 PMID:28775320 PMID:20650899 PMID:25918360

• The gene encodes multiple isoforms: isoform 1 is the full-length functional channel; isoform 2 lacks channel activity; isoform 3 acts as a negative regulator of isoform 1 and protects against oxidative stress-induced cell death.

• Subcellular localization includes the cell membrane, lysosomes, cytoplasmic vesicles, and cell projections (e.g., filopodia), consistent with its roles in ion flux and organellar signaling.

• TRPM2 is highly expressed in the brain and peripheral blood cells (especially neutrophils), with additional detection in bone marrow, spleen, heart, liver, and lung.

• The channel contributes to diverse Ca2+-dependent processes, including insulin secretion, innate immune responses (e.g., phagocytosis and cytokine release), dendritic cell differentiation, and cytoskeletal reorganization. Related references: PMID:22493272 PMID:27068538

• It mediates lysosomal Zn2+ release upon reactive oxygen species (ROS) stimulation and confers susceptibility to cell death under oxidative stress. Related references: PMID:12594222 PMID:25562606 PMID:27068538

• UniProt keywords include calcium channel, sodium channel, ion transport, phosphoprotein, disulfide bond, and transmembrane helix, reflecting its channel structure and regulation.

Experimental Guidance and Technical Tips

• The immunogen covers the C-terminal region (aa 1204–1503) that includes the Nudix domain critical for ADP-ribose binding; this polyclonal antibody may recognize epitopes conserved across isoforms 1–3.
• For western blotting, the predicted molecular weight is ~171 kDa; consider using low-percentage SDS-PAGE gels and extended transfer times for efficient detection of this large membrane protein.
• For immunofluorescence (IF-P), anticipate cell membrane, cytoplasmic vesicle, and organelle staining patterns; validate with appropriate controls in your cell line of interest.
• When using ELISA, standard indirect protocols should be optimized; the antibody cross-reacts with mouse and rat TRPM2, enabling multispecies experimental designs.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated source for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and ancillary reagents to support ion channel and oxidative stress research. This TRPM2 antibody exemplifies our capability to deliver high-quality tools for advanced cell signaling studies. From bulk procurement of routine reagents to sourcing rare-target raw materials, CamelBio bridges research concepts and clinical applications with reliable supply solutions.