Anti-TRPM1 Rabbit Polyclonal Antibody for WB and ELISA - Q7Z4N2

Core Product Specifications and Parameters

Biological Background: TRPM1 Function and Localization

• TRPM1, also known as Melastatin-1 or Long transient receptor potential channel 1 (LTrpC1), encodes a constitutively active nonselective cation channel that mediates the influx of divalent cations including Ca²⁺, Mg²⁺, Mn²⁺, Ba²⁺, and Ni²⁺, thereby promoting membrane depolarization. Related references: PMID:11535825, PMID:19436059, PMID:21278253
• In the retina, TRPM1 is critical for the depolarizing light response of ON bipolar cells. In darkness, glutamate-bound GRM6 activates G proteins that inhibit TRPM1; light decreases glutamate, relieving inhibition and allowing cation influx. Related references: PMID:19878917, PMID:19896109
• TRPM1 has been implicated in metastasis suppression; its expression is high in benign melanocytic nevi but lost in metastatic melanoma. Related references: PMID:9537257
• Alternative splicing of the human TRPM1 gene produces multiple isoforms that may differ in tissue distribution and functional properties.
• The TRPM1 protein is found in the cell membrane, endoplasmic reticulum membrane, and axonal projections, consistent with roles in both signaling and trafficking.
• TRPM1 belongs to the melastatin subfamily of TRP channels and can form heteromeric complexes with TRPM3, expanding its ionic permeability to include zinc when co‑expressed.
• Mutations in TRPM1 cause congenital stationary night blindness type 1C (CSNB1C), a non‑progressive disorder characterized by impaired night vision, highlighting its essential role in visual transduction.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C‑terminal intracellular domain (aa 1460–1540) of human TRPM1; this region may be involved in protein‑protein interactions, so consider potential competition with native binding partners during detection.
• For Western blot, expect a band at approximately 182 kDa; validate in human retinal tissue, melanocyte cell lines, or other TRPM1‑expressing samples.
• This polyclonal antibody may be suitable for ELISA; optimization of coating antigen concentration and detection conditions is recommended.
• Due to the polyclonal nature, evaluate lot‑to‑lot consistency and always include appropriate loading controls and fresh lysates to prevent degradation artifacts in your assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with one‑stop access to IVD raw materials, including validated anti‑TRPM1 polyclonal antibodies for vision and oncology research. Our comprehensive supply chain delivers optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare‑target raw‑material sourcing, streamlining your assay development from concept to clinic. Partner with CamelBio for reliable, scalable access to critical reagents that drive diagnostic kit and laboratory assay innovation.