Anti-TRPC6 Polyclonal Antibody for WB, IHC-P, ELISA - Q9Y210

Core Product Specifications and Parameters

Biological Background: TRPC6 Function and Localization

• TRPC6 (Transient receptor potential cation channel subfamily C member 6) is a member of the short transient receptor potential channel (TRPC) subfamily and exists in multiple isoforms due to alternative splicing.
• It functions as a non-selective, calcium-permeable cation channel that mediates calcium influx in response to various stimuli. Related references: PMID:19936226 PMID:23291369 PMID:26892346
• TRPC6 is directly activated by diacylglycerol (DAG) produced via phospholipase C (PLC)-mediated hydrolysis of phosphatidylinositides downstream of Gq-coupled receptors or receptor tyrosine kinases. Related references: PMID:26892346
• Unlike some TRP channels, TRPC6 is not activated by depletion of intracellular calcium stores. Related references: PMID:9930701
• In the retina, TRPC6 contributes to light-induced depolarization of intrinsically photosensitive retinal ganglion cells (ipRGCs) by mediating melanopsin-driven Gq-PLC signaling, likely as part of heteromeric TRPC6-TRPC7 channels.
• TRPC6 localizes to the cell membrane and is highly expressed in placenta, lung, spleen, ovary, small intestine, and podocytes of the kidney glomerular slit diaphragm.
• Mutations in TRPC6 are associated with focal segmental glomerulosclerosis (FSGS2), highlighting its critical role in podocyte function and renal filtration.
• Post-translational modifications include glycosylation and phosphorylation, and the protein contains ankyrin repeats and six transmembrane helices.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide within the cytoplasmic domain (amino acids 466–565), which may be conserved across species, supporting broad reactivity.
• For Western blot (WB): The predicted molecular weight is ~106 kDa; consider using reducing conditions and validate band specificity in relevant cell lysates (e.g., kidney, lung).
• For immunohistochemistry (IHC-P): Antigen retrieval methods may be required; validate staining in tissues known to express TRPC6 (e.g., placenta, podocytes).
• For ELISA: The antibody can be used as a capture or detection reagent; confirm linearity and sensitivity with recombinant TRPC6 or relevant samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a single point of access to high-quality IVD antibodies and reagents. This anti-TRPC6 polyclonal antibody supports research into calcium signaling, renal disease, and ion channel biology. Beyond individual antibodies, we offer validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets—streamlining your path from concept to clinic.