Anti-TRPC5 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9UL62

Core Product Specifications and Parameters

Biological Background: TRPC5 Function and Localization

• TRPC5 (Short transient receptor potential channel 5) is a member of the TRP family of cation channels, forming receptor-activated non-selective calcium-permeant channels. Related references: PMID:16284075, PMID:38959890, PMID:37137991
• It mediates calcium-dependent phosphatidylserine externalization and apoptosis in neurons through interaction with phospholipid scramblase 1 (PLSCR1) (By similarity).
• TRPC5 acts on distinct neuronal populations in the hypothalamus to regulate innate behaviors such as feeding, anxiety, socialization, and maternal care (By similarity).
• The channel localizes to the cell membrane and is predominantly expressed in the brain, with higher levels in fetal brain, cerebellum, and occipital pole.
• TRPC5 contains ankyrin repeats and is involved in calcium transport, with annotations highlighting its role in both calcium and zinc binding, disulfide bond formation, and glycosylation.
• The protein is associated with disease variants and is a key player in neuronal signaling and ion homeostasis.

Experimental Guidance and Technical Tips

• The immunogen was designed from a C‑terminal region (amino acids 800–900) of human TRPC5; verify that the antibody recognizes your target isoform in the relevant species.
• For Western blotting, a band near 111 kDa is expected; consider optimizing gel percentage and transfer conditions for high‑molecular‑weight proteins.
• In immunofluorescence, test both fixation and permeabilization protocols to preserve epitope integrity; co‑staining with membrane markers may aid localization studies.
• For ELISA applications, the antibody can be used as either capture or detection reagent; titration and buffer optimization are recommended.
• Cross‑reactivity with mouse suggests epitope conservation; always confirm performance in the specific tissue or cell line of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive B2B sourcing partner for IVD raw materials, offering validated antibody pairs, optimized rabbit polyclonal antibodies like this anti‑TRPC5, and bulk ancillary reagents. From ion channel research to neurological assay development, we streamline procurement of rare‑target raw materials, helping diagnostic manufacturers and researchers move seamlessly from concept to clinic.