Anti-TRPC4 Polyclonal Antibody for WB & ELISA - Q9UBN4

Core Product Specifications and Parameters

Biological Background: TRPC4 Function and Localization

• TRPC4, also known as short transient receptor potential channel 4 or Trp-related protein 4 (hTrp-4, hTrp4), is encoded by the TRPC4 gene in humans.
• It functions as a receptor-activated, non-selective calcium-permeable cation channel, facilitating calcium ion entry across the plasma membrane. Related references: PMID:11042129, PMID:11713258, PMID:16144838
• In endothelial cells, TRPC4 mediates cell-cell contact-dependent calcium entry, playing a role in endothelial signaling. Related references: PMID:19996314
• TRPC4 can form homomeric channels or heteromerize with TRPC1; the heteromeric channel has reduced calcium permeability, and the channel is also permeable to monovalent ions such as sodium, lithium, and cesium. Related references: PMID:39478185
• The protein localizes to the cell membrane, consistent with its function as an ion channel.
• Tissue specificity: strongly expressed in placenta; lower expression in heart, pancreas, kidney, and brain; present in endothelial cells. The alpha isoform predominates, and isoform beta is absent from pancreas and brain.
• Structural features include ankyrin repeats, a coiled-coil domain, disulfide bonds, and phosphorylation sites; alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• For Western blotting, consider optimizing the dilution factor; the predicted molecular weight is approximately 112 kDa. Validate the antibody in the relevant sample system, as the polyclonal nature may exhibit batch variability.
• The antibody's reactivity with mouse samples suggests utility in transgenic models; however, validation in the chosen mouse tissue or cell line is recommended.
• For ELISA, the antibody may be used in antigen capture or detection formats; rigorous optimization of coating and concentration is advised.
• Due to the polyclonal source, confirm specificity through appropriate controls and consider affinity purification requirements.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. For TRPC4-related research, we can support your development pipeline with high-quality antibodies and technical expertise. Our supply capabilities extend to rare target sourcing, ensuring you find the right materials for your diagnostic needs.