Anti-TRPC3 Polyclonal Antibody for WB, ELISA - Q13507

Core Product Specifications and Parameters

Biological Background: TRPC3 Function and Localization

• The protein is known as short transient receptor potential channel 3, also designated transient receptor protein 3 (TRP-3, hTrp-3, hTrp3), encoded by the TRPC3 (TRP3) gene. It belongs to the transient receptor potential cation channel subfamily C.
• Functions as a receptor-activated, non-selective calcium-permeant cation channel, likely operated by a phosphatidylinositol second messenger system downstream of receptor tyrosine kinases or G protein-coupled receptors. Related references: PMID:29726814, PMID:30139744, PMID:35051376
• Localizes to the cell membrane, where it mediates calcium influx important for cellular signaling cascades.
• Characterized by the presence of ankyrin (ANK) repeats, metal-binding domains, and potential glycosylation sites; alternative splicing generates multiple isoforms.
• Tissue expression is highest in the brain, with much lower levels detected in ovary, colon, small intestine, lung, prostate, placenta, and testis.
• Disease-associated variants in TRPC3 have been linked to spinocerebellar ataxia type 41 (SCA41), underscoring its role in neurological function and neurodegeneration.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning residues 500–600, a region that may contribute to specific recognition of TRPC3 over other TRP family members; cross-adsorption may further reduce off-target binding.
• For Western blotting, expect a band near 106 kDa; consider that post-translational modifications (e.g., glycosylation) or alternative splicing could shift the apparent molecular weight.
• Validate antibody performance in relevant sample systems (e.g., brain tissue lysates for strong signal, or transfected cell lines for specificity control) using appropriate loading controls.
• TRPC3 expression levels may be low in non-neuronal tissues; enrichment or longer exposure might be necessary for detection in mouse or rat non-brain samples.
• For ELISA, confirm coating conditions and blocking buffers experimentally with recombinant TRPC3 or peptide standards to optimize signal-to-noise ratios.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research teams by providing a one-stop portfolio of IVD raw materials, technical services, and consulting—from concept to clinic. This TRPC3 antibody addresses neuroscience and ion-channel research needs, and we can also supply validated antibody pairs, optimized recombinant proteins, and bulk ancillary reagents. For rare or custom targets, our sourcing service helps streamline procurement without compromising quality.