Anti-TRPC1 Polyclonal Antibody for WB, IHC-P, ELISA - P48995

Core Product Specifications and Parameters

Biological Background: TRPC1 Function and Localization

• TRPC1, also known as transient receptor protein 1 (TRP-1) or HTRP-1, is a member of the short transient receptor potential channel family.
• It forms a receptor-activated non-selective calcium-permeant cation channel, permeable to calcium and monovalent ions like sodium, lithium, and cesium. Related references: PMID:11139478 PMID:15016832 PMID:39478185
• TRPC1 can heteromerize with TRPC4 or TRPC5, resulting in channels with reduced calcium permeability compared to homomeric TRPC4/5 channels. Related references: PMID:11139478 PMID:15016832 PMID:39478185
• The channel is also activated by intracellular calcium store depletion.
• TRPC1 localizes to the cell membrane.
• It appears to be ubiquitously expressed across tissues.
• TRPC1 is characterized by ankyrin repeats, undergoes alternative splicing, and is regulated by phosphorylation; it contains disulfide bonds and zinc-binding sites.

Experimental Guidance and Technical Tips

• For Western blot, the predicted molecular weight is approximately 91 kDa; consider using a gel percentage and transfer conditions suitable for this size range.
• In immunohistochemistry (paraffin), validate the antibody on appropriate tissue sections; TRPC1’s ubiquitous expression suggests many tissues may serve as positive controls.
• As the immunogen is a synthetic peptide, the antibody may recognize specific isoforms; use appropriate controls to confirm specificity in your system.
• For calcium signaling studies, consider co-immunoprecipitation or native gel electrophoresis to examine TRPC1 heteromerization with TRPC4 or TRPC5.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research institutes, and laboratories with integrated access to high-quality IVD raw materials. For ion channel and calcium signaling targets like TRPC1, we offer validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents to support your assay development. Leverage our sourcing expertise to secure rare-target raw materials and accelerate your concept-to-clinic journey.