Anti-TRPA1 Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - O75762

Core Product Specifications and Parameters

Biological Background: TRPA1 Function and Localization

• TRPA1 (Transient receptor potential cation channel subfamily A member 1) is also known as ANKTM1, p120, and the wasabi receptor. It belongs to the TRPA subfamily of transient receptor potential channels.
  Related references:
  PMID:17259981
  PMID:21195050
  PMID:21873995
• Functions as a ligand-activated, Ca²⁺-permeable nonselective cation channel with a key role in pain detection, cold perception, cough, itch, and inner ear function.
  Related references:
  PMID:17259981
  PMID:21195050
  PMID:21873995
  PMID:19202543
• Displays high Ca²⁺ selectivity over monovalent cations and mediates membrane depolarization upon activation.
  Related references:
  PMID:19202543
  PMID:21195050
• Activated by a wide range of structurally diverse electrophilic compounds (e.g., allyl isothiocyanate, cinnamaldehyde) via covalent modification of N‑terminal cysteine residues.
  Related references:
  PMID:17164327
  PMID:20547126
  PMID:25389312
  PMID:27241698
  PMID:31866091
• Non‑electrophilic agonists bind at distinct sites in the transmembrane domain to promote channel opening.
  Related references:
  PMID:33152265
• Also functions as an ionotropic cannabinoid receptor, being activated by Δ⁹‑tetrahydrocannabinol (THC).
  Related references:
  PMID:25389312
• May serve as a mechanosensitive transduction channel in inner ear hair cells, contributing to sound perception (by similarity).
• Localizes to the cell membrane and contains multiple ANK repeats; regulated by oxidation and hydroxylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 900–1000 within the C‑terminal region of human TRPA1; verify target specificity using appropriate positive and negative controls.
• For Western blot, an expected band near 128 kDa should be observed under reducing conditions; use validated positive lysates (e.g., TRPA1‑expressing cell lines).
• For immunohistochemistry (IHC‑P) and immunofluorescence (IF‑P), antigen retrieval and permeabilization steps may be required due to the membrane localization of TRPA1.
• Cross‑reactivity with mouse and rat orthologs is reported; validate in the relevant species models before experimentation.
• Optimal antibody dilution and incubation conditions must be determined empirically by the end user.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated sourcing partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Whether you are developing pain‑related diagnostics or conducting basic ion channel research, our TRPA1 rabbit polyclonal antibody provides a reliable reagent backed by supply‑chain expertise from concept to clinic.