Anti-TROVE2/SSA2 Polyclonal Antibody for WB and ELISA - P10155

Core Product Specifications and Parameters

Biological Background: TROVE2/SSA2 (RO60) Function and Localization

• Also known as 60 kDa SS-A/Ro ribonucleoprotein, Ro60 autoantigen, and Sjoegren syndrome antigen A2, TROVE2 is a target of autoantibodies in systemic lupus erythematosus and Sjögren's syndrome.
• Functions as an RNA-binding protein that recognizes misfolded non-coding RNAs, pre-5S rRNA, and Y RNAs, and also binds endogenous Alu retroelements induced by type I interferon, modulating inflammatory cytokine secretion. Related references: PMID:18056422, PMID:26382853
• Plays a role in regulating the expression of Alu retroelements and inflammatory genes, linking it to innate immune responses. Related references: PMID:26382853
• Primarily localized to the cytoplasm, consistent with its function in cytoplasmic RNA surveillance.
• May be involved in cilia formation and/or maintenance (by similarity).
• Subject to post-translational modifications including acetylation and phosphorylation, and contains metal-binding sites, reflecting its diverse regulatory roles.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-260) of human TROVE2; thus, the polyclonal antibody may recognize epitopes in this region, suitable for both native and denaturing conditions.
• For Western blotting, the expected molecular weight is approximately 61 kDa; consider using whole-cell lysates from human, mouse, or rat tissues and validate with appropriate controls.
• In ELISA, optimize the coating antigen concentration and antibody dilution to achieve desired sensitivity; note that the polyclonal nature may require careful titration.
• Cross-reactivity with human, mouse, and rat has been indicated; verify reactivity in your specific sample system if using other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This TROVE2/SSA2 polyclonal antibody supports research into autoimmune conditions and RNA biology. Beyond antibodies, we offer validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary materials, and rare target sourcing to streamline your assay development.