Anti-Troponin I2 (TNNI2) Rabbit Monoclonal Antibody for WB, IF-P, ELISA - P48788

Core Product Specifications and Parameters

Biological Background: Troponin I2 (TNNI2) Function and Localization

• TNNI2 encodes the fast skeletal muscle isoform of the troponin I family, which also includes slow skeletal (TNNI1) and cardiac (TNNI3) isoforms.
• It functions as the inhibitory subunit of the troponin complex, regulating calcium-dependent striated muscle contraction by blocking actomyosin ATPase activity.
• TNNI2 undergoes alternative splicing and post-translational modifications including acetylation and phosphorylation, which may modulate its regulatory properties.
• The protein is localized to the thin filaments of sarcomeres in fast-twitch skeletal muscle fibers, where it interacts with actin and other troponin subunits.
• Mutations in TNNI2 are associated with distal arthrogryposis, a congenital disorder characterized by joint contractures.
• Structural studies have elucidated its actin-binding interface and regulatory mechanism, underpinning its classification as a reference proteome member.

Experimental Guidance and Technical Tips

• For Western blotting, expect a band around 21 kDa; use reducing conditions and validate detection in skeletal muscle lysates or relevant cell lines.
• Consider including mouse and rat positive controls to confirm cross-reactivity; titrate antibody concentration to optimize signal-to-noise ratio.
• For immunofluorescence (IF-P), permeabilize cells or tissue sections adequately; co-stain with sarcomeric markers to confirm myofibrillar localization.
• ELISA applications may require pairing with a suitable capture antibody; perform checkerboard titrations to determine optimal working concentrations.
• Since this is a rabbit monoclonal antibody, use an appropriate anti-rabbit secondary antibody for detection, and include appropriate negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials, featuring validated antibody pairs and rare-target sourcing capabilities. This Troponin I2 (TNNI2) rabbit mAb exemplifies our commitment to supplying high-specificity reagents for muscle biology and myopathy research. From bulk monoclonal antibodies to ancillary reagents, we streamline procurement so you can accelerate assay development and biomarker discovery.