Anti-Troponin I1/TnI1 Monoclonal Antibody for WB, ELISA - P19237

Core Product Specifications and Parameters

Biological Background: Troponin I1/TnI1 Function and Localization

• Troponin I1 (TNNI1) is the inhibitory subunit of the troponin complex, regulating calcium-dependent muscle contraction in striated muscle.
• It is the slow-twitch isoform of troponin I, predominantly expressed in slow skeletal muscle fibers and essential for sustained contractile activity.
• Highest expression levels are observed in human skeletal muscle tissues (e.g., gastrocnemius) and differentiated primary human muscle cells.
• The protein directly binds actin and remains associated with the thin filament, conferring calcium sensitivity to actomyosin ATPase activity.
• Post-translational modifications include acetylation and phosphorylation, which may modulate its function and interactions.
• Identified as a reference proteome component, TNNI1 is a well-characterized muscle protein with established biomarker potential.

Experimental Guidance and Technical Tips

• The immunogen comprises the full-length human Troponin I1 protein (aa 1-187), suggesting the antibody can detect both native and denatured forms; suitable for WB under reducing conditions.
• For Western blot, expect a band at ~22kDa; optimize loading with human skeletal muscle or rat muscle lysates.
• For ELISA, pair with a suitable detection antibody and validate using recombinant protein or tissue lysates; consider rat samples given cross-reactivity.
• Cross-reactivity with rat enables translational research; verify performance in intended experimental models.
• Not validated for IHC or IF; if required, perform additional optimization and validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides one-stop sourcing for IVD raw materials, delivering validated monoclonal antibodies like this Troponin I1 antibody to support muscle biology and diagnostic R&D. Our supply chain ensures reliable access to bulk quantities, ancillary reagents, and rare-target raw materials, streamlining your development from concept to clinic.