Anti-Tropomyosin 1 Rabbit Monoclonal Antibody for WB, IP, IF-P, IHC-P, ELISA - P09493

Core Product Specifications and Parameters

Biological Background: Tropomyosin alpha-1 chain Function and Localization

• Tropomyosin alpha-1 chain binds to actin filaments and, together with the troponin complex, regulates calcium-dependent striated muscle contraction (PubMed:23170982). Related references: PMID:23170982
• In smooth muscle, contraction is regulated via interaction with caldesmon; in non-muscle cells, it stabilizes cytoskeleton actin filaments.
• The protein localizes to the cytoplasm and cytoskeleton, and exhibits tissue-specific isoform expression: isoform 1 in skeletal/cardiac muscle, isoform 10 only in cardiac tissue; also detected in breast cancer samples.
• Post-translational modifications include acetylation and phosphorylation, and the protein features a coiled-coil structure. It is associated with cardiomyopathy and acts as a disease variant.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 185–284; for applications such as WB, IF-P, and IHC-P, consider optimizing antigen retrieval and blocking conditions in your specific sample system.
• This antibody is recommended for WB, IP, IF-P, IHC-P, and ELISA; validate it in the relevant species (human, mouse, rat) to confirm performance.
• As a rabbit monoclonal antibody, it may offer batch-to-batch consistency; for quantitative assays, titrate appropriately.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing solution for IVD raw materials, from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents and rare-target sourcing. This Tropomyosin 1 antibody supports research into muscle contraction, cytoskeletal dynamics, and cardiac/skeletal muscle biology, helping diagnostic manufacturers accelerate development from concept to clinic.