Anti-Tropomyosin 1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P09493

Core Product Specifications and Parameters

Biological Background: Tropomyosin alpha-1 chain Function and Localization

• Tropomyosin alpha-1 chain (TPM1) is a coiled-coil protein that binds actin filaments in muscle and non-muscle cells and, in association with the troponin complex, mediates calcium-dependent regulation of vertebrate striated muscle contraction. Related references: PMID:23170982
• In smooth muscle, TPM1 regulates contraction via interaction with caldesmon.
• In non-muscle cells, TPM1 stabilizes the cytoskeletal actin network.
• Subcellular localization is primarily cytoplasmic, associated with the cytoskeleton.
• Tissue specificity: detected in primary breast cancer tissues but not normal breast; isoform 1 is expressed in adult and fetal skeletal and cardiac muscle (higher in cardiac), while isoform 10 is cardiac-specific.
• Post-translational modifications include acetylation and phosphorylation, as identified by proteomics.
• Alternative splicing generates multiple tissue-specific isoforms; mutations in TPM1 are linked to cardiomyopathy.

Experimental Guidance and Technical Tips

• The immunogen (aa 1-245) corresponds to the N-terminal region conserved across many TPM1 isoforms; verify isoform detection in your specific sample context.
• Western blot: expect a predominant band at ~33 kDa; additional bands from isoforms or post-translational modifications may appear. Validate with a relevant positive control lysate.
• Immunohistochemistry (IHC-P): standard antigen retrieval protocols (e.g., heat-induced epitope retrieval) are recommended; optimize primary antibody dilution on control tissues.
• Immunofluorescence (IF/ICC): include a permeabilization step to access cytoplasmic cytoskeletal targets; counterstain with phalloidin to confirm actin colocalization.
• ELISA: suitable for both direct and indirect detection; optimal coating concentration should be determined experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with streamlined access to validated IVD raw materials. We supply optimized polyclonal antibodies, bulk ancillary reagents, and expert support for rare or custom targets. Our Tropomyosin 1 antibody supports cytoskeleton and muscle biology research, helping you move from concept to clinic with confidence.