Anti-TROP-2 Rabbit Monoclonal Antibody for WB - P09758

Core Product Specifications and Parameters

Biological Background: TROP-2 Function and Localization

• TROP-2 is a cell surface glycoprotein encoded by the TACSTD2 gene, also known as Tumor-associated calcium signal transducer 2, EGP-1, or GA733-1.
• It may function as a growth factor receptor, playing a role in intracellular calcium signaling.
• TROP-2 is a transmembrane protein located on the cell membrane, with a single transmembrane helix and an extracellular domain containing disulfide bonds.
• The protein is classified as a tumor antigen and is overexpressed in various carcinomas, including pancreatic carcinoma cell lines.
• TROP-2 is expressed in normal placenta and is implicated in sensory transduction and vision processes, potentially through its role in cell adhesion or signaling.
• Post-translational modifications include glycosylation, and its structure has been solved (3D-structure keyword), contributing to understanding its function.
• Mutations in TACSTD2 are associated with corneal dystrophy and amyloidosis, indicating its importance in tissue homeostasis.
• The protein's molecular weight is approximately 35.7 kDa (UniProt: 35709 Da), though the supplied antibody detects a band at 36 kDa.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein corresponding to the extracellular domain (amino acids 27-274) of human TROP-2. This domain is likely targeted for applications such as WB, IHC-P, IF/ICC, FC, and ELISA. Consider pre-validating in relevant sample systems, particularly pancreatic carcinoma cell lines or placental tissues, where expression is confirmed.
• For Western blotting, the expected band size is ~36 kDa. Use appropriate positive controls like human pancreatic cancer cell lines.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), membrane staining should be observed. Optimize antigen retrieval protocols for paraffin-embedded sections.
• The antibody's monoclonal nature ensures batch-to-batch consistency, suitable for robust assay development. Ensure proper storage (shipped on ice) and handle according to manufacturer's recommendations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For TROP-2-based assay development, we also supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Our expertise supports cancer diagnostic projects, ensuring seamless procurement and tailored technical consultation.