Anti-TROP-2 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, mIHC, ELISA - P09758

Core Product Specifications and Parameters

Biological Background: TROP-2 Function and Localization

• TROP-2, also known as tumor-associated calcium signal transducer 2, is encoded by the TACSTD2 gene and is also referred to as cell surface glycoprotein Trop-2, membrane component chromosome 1 surface marker 1 (M1S1), and pancreatic carcinoma marker protein GA733-1.
• It was originally identified as a cell surface glycoprotein highly expressed in placental trophoblasts and pancreatic carcinoma cell lines, and subsequently characterized as a tumor antigen.
• TROP-2 is a transmembrane protein that may function as a growth factor receptor, potentially transducing calcium signals and regulating cell proliferation.
• The protein contains a signal peptide, an extracellular domain rich in disulfide bonds and N-linked glycosylation sites, a single transmembrane helix, and a short cytoplasmic tail.
• Subcellular localization: It is primarily localized to the plasma membrane.
• Key post-translational modifications include glycosylation, which contributes to the observed molecular weight of ~36 kDa (theoretical MW ~35.7 kDa).
• TROP-2 is implicated in several pathological conditions, including amyloidosis and certain corneal dystrophies, as noted by its keyword associations.
• Due to its high and selective expression in many epithelial cancers, TROP-2 is a validated tumor antigen and a target for antibody-based diagnostics and therapeutics.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fragment (amino acids 27-274) covering the entire extracellular domain of human TROP-2, suggesting the antibody likely recognizes the extracellular region; consider its suitability for surface staining in live cells and formalin-fixed, paraffin-embedded (FFPE) tissues.
• For Western blotting (WB), validate the antibody in whole cell lysates from human placental tissue, pancreatic carcinoma, or TROP-2-positive cancer cell lines; expect a band at ~36 kDa, with possible minor shifts due to glycosylation.
• For immunohistochemistry (IHC-P), antigen retrieval may be required; optimize citrate or EDTA-based protocols to unmask the epitope in FFPE sections.
• For multiplex immunohistochemistry (mIHC), ensure compatibility with your detection system and other antibody panels; confirm staining specificity through appropriate controls.
• For ELISA and immunofluorescence (IF-P), titrate the antibody and validate in the relevant sample system; non-specific binding can be reduced by appropriate blocking.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD manufacturers, laboratories, and research institutes, delivering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material procurement. This TROP-2 rabbit monoclonal antibody supports developers working on cancer diagnostics, particularly for carcinomas where TROP-2 is overexpressed. Whether you need bulk antibody supplies, technical consultation, or complementary raw materials, CamelBio streamlines your path from concept to clinic.