Anti-TRNT1 Polyclonal Antibody for WB, ELISA - Q96Q11

Core Product Specifications and Parameters

Biological Background: TRNT1 Function and Localization

• TRNT1 is a nucleotidyltransferase that catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs, which is required for amino acid attachment, using CTP and ATP as substrates. Related references: PMID:11504732, PMID:25193871, PMID:25640237
• It mediates tRNA repair and recycling downstream of ribosome-associated quality control (RQC) by adding the CCA sequence after ANKZF1 cleavage and ELAC1 repair. Related references: PMID:31011209
• TRNT1 also functions in tRNA surveillance by adding a tandem CCACCA sequence to the 3' terminus of unstable tRNAs, marking them for rapid degradation, while stable tRNAs receive only a single CCA. Related references: PMID:22076379, PMID:25640237
• An isoform exists that adds only two C residues (CC-) to the 3' terminus instead of a complete CCA end.
• Subcellular localization includes the mitochondrion, cytoplasm, and nucleus.
• The protein contains an ATP-binding site, requires magnesium as a cofactor, and is subject to acetylation and phosphorylation.
• Mutations in TRNT1 are associated with retinitis pigmentosa and other disorders, as indicated by disease variant annotations.

Experimental Guidance and Technical Tips

• This polyclonal antibody is raised against a recombinant fragment (amino acids 135-434) and may recognize multiple isoforms or processed forms of TRNT1; consider validation by peptide blocking or knockdown.
• For western blot (WB), the predicted molecular weight is ~50 kDa, but actual migration may vary depending on post-translational modifications or sample conditions.
• For ELISA applications, a checkerboard titration is recommended to determine optimal coating antigen and antibody dilutions.
• Validate reactivity in relevant sample systems, such as tissues or cell lines expressing TRNT1 (human, mouse, or rat).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, providing integrated access to antibodies, technical services, and consulting from concept to clinic. This anti-TRNT1 polyclonal antibody supports research in tRNA processing, mitochondrial biology, andretinitis pigmentosa. We offer a comprehensive portfolio including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and sourcing for rare target raw materials, helping diagnostic manufacturers and labs streamline their development workflows.