Anti-TRMT61A Rabbit Monoclonal Antibody for WB, IF, IHC, ELISA - Q96FX7

Core Product Specifications and Parameters

Biological Background: TRMT61A Function and Localization

• TRMT61A is the catalytic subunit of tRNA (adenine-N(1)-)-methyltransferase, which catalyzes the formation of N(1)-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA. Related references: PMID:16043508
• It also serves as the catalytic subunit of the mRNA N(1)-methyltransferase complex, mediating methylation of adenosine residues at the N(1) position in a small subset of mRNAs with tRNA T-loop-like structures, occurring at low stoichiometries. Related references: PMID:29072297, PMID:29107537
• The protein is also known as GCD14, TRM61, Gcd14p, hTRM61, and C14orf172, and belongs to the TRM61 family of methyltransferases.
• It localizes primarily to the nucleus, consistent with its role in tRNA and mRNA processing.
• Post-translational modifications include acetylation and phosphorylation, as indicated by proteomics studies.
• It is a conserved protein with a molecular weight of approximately 31-32 kDa (calculated 31,382 Da).

Experimental Guidance and Technical Tips

• The immunogen consists of a recombinant protein spanning amino acids 1-289 of human TRMT61A, which may recognize the full-length and major isoforms. Validate expression in your target cell lines or tissues before performing quantitative studies.
• For Western blot (WB), a band around 31 kDa is expected. Optimize loading controls and antibody dilutions for your system, as detection may vary depending on sample type and preparation.
• In immunofluorescence (IF), TRMT61A is typically nuclear; consider using nuclear counterstains (e.g., DAPI) to confirm localization. Permeabilization protocols should be validated to ensure antibody accessibility.
• Cross-reactivity with mouse and rat has been reported, making this antibody suitable for translational studies in rodent models. However, always verify reactivity in your specific application and species.
• This rabbit monoclonal antibody offers batch-to-batch consistency and is suitable for applications including WB, IF/ICC, IF-P, IHC-P, and ELISA. Appropriate isotype controls should be included in all experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a comprehensive one-stop platform for IVD raw materials, technical services, and consulting—supporting projects from concept to clinic. This anti-TRMT61A antibody exemplifies our commitment to providing high-quality reagents for epitranscriptomics and RNA modification research. Beyond this product, we can source validated antibody pairs, bulk monoclonal/polyclonal antibodies, ancillary reagents, and custom raw materials for rare targets—streamlining your supply chain and accelerating development.