Anti-TRMT61A Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - Q96FX7

Core Product Specifications and Parameters

Biological Background: TRMT61A Function and Localization

• TRMT61A (also known as GCD14, TRM61, hTRM61, C14orf172) is the catalytic subunit of the tRNA (adenine(58)-N(1))-methyltransferase, responsible for incorporating N(1)-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA. Related references: PMID:16043508
• It also functions as the catalytic subunit of an mRNA N(1)-methyltransferase complex, mediating adenosine methylation at the N(1) position in a small subset of mRNAs, particularly within tRNA T-loop-like structures. Related references: PMID:29072297 PMID:29107537
• The protein localizes predominantly to the nucleus, consistent with its roles in tRNA and mRNA processing.
• TRMT61A is a member of the methyltransferase family and utilizes S-adenosyl-L-methionine (SAM) as a methyl donor, characteristic of RNA methyltransferases.
• Post-translational modifications reported include phosphorylation and acetylation, suggesting regulatory control of its activity.
• As a key enzyme in RNA modification, TRMT61A contributes to the proper folding, stability, and function of tRNAs and potentially impacts translation initiation fidelity.
• The human TRMT61A gene encodes a protein of approximately 31 kDa (calculated molecular weight 31382 Da) and shares functional orthologs in mouse and rat.
• No tissue-specific expression data is currently available, indicating likely ubiquitous expression with potential cell-type-specific regulatory roles.

Experimental Guidance and Technical Tips

• The immunogen spans the full-length human TRMT61A protein (amino acids 1-289), so the antibody may recognize both unmodified and post-translationally modified forms. For WB, a band around 31 kDa is expected; additional bands could represent modified species or degradation products.
• For IF/ICC, nuclear enrichment should be observed; permeabilization and fixation conditions should be optimized for the specific cell type.
• In IHC-P, antigen retrieval may be necessary; validation with tissue microarrays or known positive/negative controls is recommended.
• This polyclonal antibody is suitable for ELISA-based detection of TRMT61A in cell or tissue lysates; titration and appropriate blocking reagents should be empirically determined.
• The reported cross-reactivity with human, mouse, and rat suggests conserved epitopes; however, testing in the target species and biological system is essential for any novel application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers by offering a reliable supply of high-quality raw materials, including this TRMT61A polyclonal antibody for RNA modification and methylation studies. Whether you need validated antibody pairs, optimized monoclonal antibodies, or bulk ancillary reagents, our one-stop platform accelerates your development process and ensures seamless access to rare targets and customized sourcing solutions.