Anti-TRMT2B Rabbit pAb for WB, IHC-P, ELISA - Q96GJ1

Core Product Specifications and Parameters

Biological Background: TRMT2B Function and Localization

• TRMT2B (tRNA (uracil-5-)-methyltransferase homolog B) is a mitochondrial methyltransferase that catalyzes the formation of 5-methyluridine at position 54 in tRNAs (m5U54) and at position 429 in 12S rRNA (m5U429), utilizing S-adenosyl-L-methionine as a cofactor (PubMed:31948311, PubMed:34556860). Related references: PMID:31948311, PMID:34556860
• The enzyme localizes to the mitochondrial matrix, consistent with its role in mitochondrial RNA modification.
• Also known as TRM2 homolog B, the protein is encoded by the TRMT2B gene (previously CXorf34) and is subject to alternative splicing, producing multiple isoforms.
• TRMT2B is involved in tRNA processing and rRNA processing, as indicated by UniProt annotation, and does not affect RNA stability or mitochondrial translation (PubMed:31948311). Related references: PMID:31948311
• The protein has a predicted molecular weight of approximately 56 kDa (56476 Da) and contains a mitochondrial transit peptide for import.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1–250 of human TRMT2B, which includes the N-terminal region; this may cover the mitochondrial targeting sequence. When designing complementary antibodies or validating epitope accessibility, consider the potential impact of mitochondrial import processing.
• For Western blotting, a band at ~56 kDa is expected; validate expression in mitochondrial-enriched fractions or whole-cell lysates from human and rat cell lines.
• In immunohistochemistry (IHC-P), antigen retrieval methods may be required; test on positive control tissues with known TRMT2B expression, and optimize primary antibody dilution.
• For ELISA, the antibody can be used as a capture or detection reagent; confirm binding specificity using recombinant TRMT2B protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw-material sourcing, providing diagnostic manufacturers, laboratories, and research institutes with a full spectrum of antibodies, antigen reagents, and technical support from early R&D to clinical application. This TRMT2B antibody supports mitochondrial biology and RNA modification research, and our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare or challenging targets. We accelerate your assay development with a one-stop, B2B-focused supply chain tailored to your project needs.